来自海洋细菌灿烂弧菌的一个隐蔽质粒的完整核苷酸序列及开放阅读框的鉴定

Complete nucleotide sequence of a cryptic plasmid from the marine bacterium Vibrio splendidus and identification of open reading frames.

作者信息

Powers L G, Mallonee J T, Sobecky P A

机构信息

School of Biology, Georgia Institute of Technology, Atlanta, Georgia, 30332-0230, USA.

出版信息

Plasmid. 2000 Jan;43(1):99-102. doi: 10.1006/plas.1999.1438.

DOI:10.1006/plas.1999.1438

PMID:10610823

Abstract

A 2.3-kb replication-proficient fragment was previously obtained from a cryptic plasmid (pPS41) isolated from a marine Vibrio splendidus isolate (P. A. Sobecky, T. J. Mincer, M. C. Chang, A. Toukdarian, and D. R. Helinski, 1998, Appl. Environ. Microbiol. 64, 2822-2830). Analysis of the complete nucleotide sequence of plasmid pPS41 revealed two additional open reading frames (ORFs). Analysis of ORF-1 revealed that its translated product has 125 amino acids with a predicted MW of 16,978 and ORF-2 encodes a putative protein of 151 amino acids with a predicted MW of 19,802. The ORF-2 encoded protein showed 31 to 35% sequence homology to proteins identified to have a role in plasmid mobilization. These proteins are encoded on plasmids found in Escherichia coli and Pasteurella multocida. Plasmid pPS41 could be mobilized by a conjugative plasmid at frequencies of 1 x 10(-2) to 2 x 10(-2).

摘要

一个2.3 kb的复制功能片段先前是从一株海洋灿烂弧菌分离株（P. A. 索贝茨基、T. J. 明瑟、M. C. 张、A. 图卡德里安和D. R. 赫林斯基，1998年，《应用与环境微生物学》64卷，2822 - 2830页）中分离得到的一个隐蔽质粒（pPS41）上获得的。对质粒pPS41完整核苷酸序列的分析揭示了另外两个开放阅读框（ORF）。对ORF - 1的分析表明，其翻译产物有125个氨基酸，预测分子量为16,978，而ORF - 2编码一个推定的含151个氨基酸的蛋白质，预测分子量为19,802。ORF - 2编码的蛋白质与已确定在质粒转移中起作用的蛋白质有31%至35%的序列同源性。这些蛋白质由存在于大肠杆菌和多杀巴斯德氏菌中的质粒编码。质粒pPS41可被一个接合性质粒以1×10⁻²至2×10⁻²的频率进行转移。