药物诱导的分枝杆菌变化的定量蛋白质组学分析

Quantitative proteomic analysis of drug-induced changes in mycobacteria.

作者信息

Hughes Minerva A, Silva Jeffrey C, Geromanos Scott J, Townsend Craig A

机构信息

Department of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA.

出版信息

J Proteome Res. 2006 Jan;5(1):54-63. doi: 10.1021/pr050248t.

DOI:10.1021/pr050248t

PMID:16396495

Abstract

A new approach for qualitative and quantitative proteomic analysis using capillary liquid chromatography and mass spectrometry to study the protein expression response in mycobacteria following isoniazid treatment is discussed. In keeping with known effects on the fatty acid synthase II pathway, proteins encoded by the kas operon (AcpM, KasA, KasB, Accd6) were significantly overexpressed, as were those involved in iron metabolism and cell division suggesting a complex interplay of metabolic events leading to cell death.

摘要

讨论了一种使用毛细管液相色谱和质谱进行定性和定量蛋白质组学分析的新方法，以研究异烟肼治疗后分枝杆菌中的蛋白质表达反应。与对脂肪酸合酶II途径的已知作用一致，kas操纵子编码的蛋白质（AcpM、KasA、KasB、Accd6）显著过表达，参与铁代谢和细胞分裂的蛋白质也是如此，这表明导致细胞死亡的代谢事件之间存在复杂的相互作用。