Uemura Akiko, Watarai Shinobu, Iwasaki Tadashi, Kodama Hiroshi
Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan.
J Vet Med Sci. 2005 Dec;67(12):1197-201. doi: 10.1292/jvms.67.1197.
The immune responses of mice against glycosphingolipid (GSL) antigens and the effect of the phospholipid composition of liposomes on the immunogenicity in mice of liposome-associated GSL antigens were examined. The immunization with GSL antigen alone was unable to induce any detectable anti-GSL antibody responses. On the other hand, the immune responses against GSL antigens were detected after immunization with liposomes composed of dipalmitoylphosphatidylcholine (DPPC) (0.5 micromol), cholesterol (Chol) (0.5 micromol), Salmonella minnesota R595 lipopolysaccharides (LPS) (10 microg) and GSL (0.05 micromol) (DPPC-liposome). However, the administration with liposome composed of dimyristoylphosphatidylcholine (DMPC) (0.5 micromol), Chol (0.5 micromol), S. minnesota R595 LPS (10 microg) and GSL (0.05 micromol) and with liposomes composed of distearylphosphatidylcholine (DSPC) (0.5 micromol), Chol (0.5 micromol), and S. minnesota R595 LPS (10 microg) and GSL (0.05 micromol) was ineffective for the induction of the immune responses against GSL antigens. These results suggest that DPPC-liposome would serve effectively as a delivery vehicle for inducing immune responses against GSL antigen.
研究了小鼠针对糖鞘脂（GSL）抗原的免疫反应以及脂质体的磷脂组成对脂质体相关GSL抗原在小鼠体内免疫原性的影响。单独用GSL抗原免疫不能诱导任何可检测到的抗GSL抗体反应。另一方面，在用由二棕榈酰磷脂酰胆碱（DPPC）（0.5微摩尔）、胆固醇（Chol）（0.5微摩尔）、明尼苏达沙门氏菌R595脂多糖（LPS）（10微克）和GSL（0.05微摩尔）组成的脂质体（DPPC-脂质体）免疫后，检测到了针对GSL抗原的免疫反应。然而，用由二肉豆蔻酰磷脂酰胆碱（DMPC）（0.5微摩尔）、Chol（0.5微摩尔）、明尼苏达沙门氏菌R595 LPS（10微克）和GSL（0.05微摩尔）组成的脂质体以及由二硬脂酰磷脂酰胆碱（DSPC）（0.5微摩尔）、Chol（0.5微摩尔）和明尼苏达沙门氏菌R595 LPS（10微克）和GSL（0.05微摩尔）组成的脂质体给药，对诱导针对GSL抗原的免疫反应无效。这些结果表明，DPPC-脂质体可有效地作为诱导针对GSL抗原免疫反应的递送载体。
