Peters J, Takahashi S, Tada M, Miyake Y
Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka.
J Biochem. 1992 May;111(5):643-8. doi: 10.1093/oxfordjournals.jbchem.a123812.
mGK-6-derived true tissue kallikrein was shown to be synthesized in mouse pituitary AtT-20 cells. This cell line, which is capable of processing other prohormones, only partially processed the proform of kallikrein to its active form, secreting it predominantly as the proform. The secretion of the active form was stimulated in response to a secretagogue, 8-bromo-cyclic AMP. These results imply that not only cellular elements capable of directing the processing of the proform to the active form and the intracellular transport of the kallikrein, but also a pathway that regulates the release of the active form may be present in the AtT-20 cells, thus the availability of this cell line for investigation of biosynthetic and secretory processes for tissue kallikrein in vivo being suggested.
已证实从小鼠促性腺激素释放激素神经元系-6（mGK-6）衍生而来的真组织激肽释放酶在小鼠垂体AtT-20细胞中合成。该细胞系能够加工其他激素原，但仅将激肽释放酶原部分加工成其活性形式，主要以酶原形式分泌。活性形式的分泌在促分泌剂8-溴环磷酸腺苷（8-bromo-cyclic AMP）的作用下受到刺激。这些结果表明，AtT-20细胞中不仅存在能够将酶原加工成活性形式并进行激肽释放酶细胞内运输的细胞成分，还可能存在调节活性形式释放的途径，因此提示该细胞系可用于研究体内组织激肽释放酶的生物合成和分泌过程。
