HPLC separation of enantiomers of some potential beta-blockers of the aryloxyaminopropanol type using macrocyclic antibiotic chiral stationary phases. Studies of the mechanism of enantioseparation, Part XI.

The macrocyclic antibiotic type chiral stationary phases based on native vancomycin, teicoplanin and teicoplanin aglycone (Chirobiotic V, Chirobiotic T and Chirobiotic TAG) were used for the HPLC separation of enantiomers of potential beta-blockers of the aryloxyaminopropanol type with a morpholino moiety in the hydrophilic part of the molecule. The chromatographic results presented include: retention, separation and resolution factors along with the enantioselective free energy difference corresponding to the separation of the enantiomers. Comparison of the results obtained on the three macrocyclic chiral stationary phases showed that in most cases the teicoplanin aglycone is responsible for enantioseparation of morpholino derivatives. By application of these chiral stationary phase highest resolution factors were achieved with a polar organic mobile phase system.