冠醚介导的离子液体中细胞色素C的萃取及功能转化

Crown ether-mediated extraction and functional conversion of cytochrome C in ionic liquids.

作者信息

Shimojo Kojiro, Nakashima Kazunori, Kamiya Noriho, Goto Masahiro

机构信息

Division of Environment and Radiation Sciences, Nuclear Science and Energy Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki 319-1195, Japan.

出版信息

Biomacromolecules. 2006 Jan;7(1):2-5. doi: 10.1021/bm050847t.

DOI:10.1021/bm050847t

PMID:16398488

Abstract

We report that a macrocyclic ligand enables transfer of a protein from an aqueous phase to ionic liquids. The extraction behavior of heme protein cytochrome c (Cyt-c) from an aqueous phase into ionic liquids was investigated with crown ethers. A hydroxyl-group-containing ionic liquid with dicyclohexano-18-crown-6 was found to be capable of quantitative partitioning of Cyt-c, whereas the protein transfer using conventional organic solvents was negligibly small. Furthermore, we clarified that Cyt-c solubilized in ionic liquids caused a structural transformation of Cyt-c, which triggers its functional conversion from an electron-transfer protein to peroxidase.

摘要

我们报道了一种大环配体能够使蛋白质从水相转移至离子液体中。使用冠醚研究了血红素蛋白细胞色素c（Cyt-c）从水相到离子液体中的萃取行为。发现含羟基的离子液体与二环己基-18-冠-6能够对Cyt-c进行定量分配，而使用传统有机溶剂时蛋白质的转移量小到可以忽略不计。此外，我们还阐明了溶解在离子液体中的Cyt-c会导致其结构转变，从而引发其从电子传递蛋白到过氧化物酶的功能转换。