Matsuda M, Tazumi A, Kagawa S, Sekizuka T, Murayama O, Moore J E, Millar B C
Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, UK.
BMC Vet Res. 2006 Jan 6;2:1. doi: 10.1186/1746-6148-2-1.
At present, six accessible sequences of 16S rDNA from Taylorella equigenitalis (T. equigenitalis) are available, whose sequence differences occur at a few nucleotide positions. Thus it is important to determine these sequences from additional strains in other countries, if possible, in order to clarify any anomalies regarding 16S rDNA sequence heterogeneity. Here, we clone and sequence the approximate full-length 16S rDNA from additional strains of T. equigenitalis isolated in Japan, Australia and France and compare these sequences to the existing published sequences.
Clarification of any anomalies regarding 16S rDNA sequence heterogeneity of T. equigenitalis was carried out. When cloning, sequencing and comparison of the approximate full-length 16S rDNA from 17 strains of T. equigenitalis isolated in Japan, Australia and France, nucleotide sequence differences were demonstrated at the six loci in the 1,469 nucleotide sequence. Moreover, 12 polymorphic sites occurred among 23 sequences of the 16S rDNA, including the six reference sequences.
High sequence similarity (99.5% or more) was observed throughout, except from nucleotide positions 138 to 501 where substitutions and deletions were noted.
目前，可获取来自马生殖道泰勒菌（T. equigenitalis）的6个16S rDNA序列，其序列差异仅存在于少数核苷酸位置。因此，如果可能的话，确定其他国家分离的额外菌株的这些序列，对于阐明16S rDNA序列异质性方面的任何异常情况很重要。在此，我们克隆并测序了从日本、澳大利亚和法国分离的马生殖道泰勒菌额外菌株的近似全长16S rDNA，并将这些序列与已发表的现有序列进行比较。
对马生殖道泰勒菌16S rDNA序列异质性的任何异常情况进行了阐明。在对从日本、澳大利亚和法国分离的17株马生殖道泰勒菌的近似全长16S rDNA进行克隆、测序和比较时，在1469个核苷酸序列的6个位点发现了核苷酸序列差异。此外，在16S rDNA的23个序列（包括6个参考序列）中出现了12个多态性位点。
除了在核苷酸位置138至501处发现有替换和缺失外，整体上观察到了高度的序列相似性（99.5%或更高）。
