Miyazawa T, Matsuda M, Isayama Y, Samata T, Ishida Y, Ogawa S, Takei K, Honda M, Kamada M
Department of Biosciences, College of Environmental Health, Azabu University, Sagamihara, Japan.
Vet Res Commun. 1995;19(4):265-71. doi: 10.1007/BF01839309.
Profiles of the genomic DNA of 104 strains of T. equigenitalis isolated from brood mares with contagious equine metritis in Hokkaido during the breeding seasons from 1980 to 1993, as well as those of five strains (SS28, EQ56, EQ59, EQ70 and HH139) previously isolated in Japan were examined after restriction digestion and crossed-field gel electrophoresis. These profiles were essentially identical to each other and the various isolates and strains appeared to have a common genotype, designated 'genotype J', with respect to two restriction enzymes, ApaI and NotI. These results suggest a common source for all these isolates obtained over the course of more than 10 years in Japan.
对1980年至1993年繁殖季节期间从北海道患有传染性马子宫内膜炎的母马中分离出的104株马生殖支原体的基因组DNA图谱，以及之前在日本分离出的5株菌株（SS28、EQ56、EQ59、EQ70和HH139）进行了限制性消化和交变电场凝胶电泳后进行了检测。这些图谱彼此基本相同，各种分离株和菌株在两种限制性内切酶ApaI和NotI方面似乎具有共同的基因型，称为“基因型J”。这些结果表明，在日本10多年间获得的所有这些分离株都有一个共同的来源。
