具有不同修复活性的鸡胚细胞中西方马脑炎病毒的自发诱变和诱发诱变。
Spontaneous and induced mutagenesis in Western Equine encephalomyelitis virus in chick embryo cells with different repair activity.
作者信息
Dubinin N P, Zasukhina G D, Nesmashnova V A, Lvova G N
出版信息
Proc Natl Acad Sci U S A. 1975 Jan;72(1):386-8. doi: 10.1073/pnas.72.1.386.
Evidence was obtained indicating differences in the survival rate of Western equine encephalomyelitis virus after exposure to ultraviolet radiation and methyl methanesulfonate in commercial and leukosisfree chick embryo cells that differed in repair activity. The levels of spontaneous mutagenesis (on the basis of the yield of small palque variants of the encephalomyelitis virus) did not essentially change when the virus was passage in leukosis-free chick embryo cells, whereas an increase in the number of small palque variants was observed in the cells of commercial chick embryos. A 10-fold increase in the number of induced virus variants was observed in commercial chick embryo cells in experiments with methyl methanesulfonate as compared with the contorl, whereas the induction of virus variants was not noted in leukosis-free cells.
有证据表明，在商业鸡胚细胞和修复活性不同的无白血病鸡胚细胞中，西部马脑脊髓炎病毒暴露于紫外线辐射和甲磺酸甲酯后，其存活率存在差异。当病毒在无白血病鸡胚细胞中传代时，自发诱变水平（基于脑脊髓炎病毒小蚀斑变体的产量）基本没有变化，而在商业鸡胚细胞中观察到小蚀斑变体数量增加。与对照组相比，在使用甲磺酸甲酯的实验中，商业鸡胚细胞中诱导病毒变体的数量增加了10倍，而在无白血病细胞中未发现病毒变体的诱导现象。
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