通过荧光素-次氮基三乙酸共轭物对含有多组氨酸序列的细胞外蛋白质进行选择性标记。
Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein-nitrilotriacetic acid conjugate.
作者信息
Goldsmith Christian R, Jaworski Jacek, Sheng Morgan, Lippard Stephen J
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, 02139, USA.
出版信息
J Am Chem Soc. 2006 Jan 18;128(2):418-9. doi: 10.1021/ja0559754.
Abstract
The compound NTA-DCF consists of two components, a dichlorofluorescein (DCF) reporter and a nitrilotriacetic acid (NTA) functionality. The latter binds polyhistidine sequences selectively through a bridging metal ion. The NTA-DCF conjugate has photophysical properties similar to those of the parent DCF fluorophore both by itself and as its nickel(II) complex. The insensitivity of the emission to paramagnetic ions allows the probe to label His6-tagged proteins fluorescently on the extracellular surfaces of HEK 293-T and HeLa cells.
摘要
化合物NTA-DCF由两部分组成，一个二氯荧光素（DCF）报告基团和一个次氮基三乙酸（NTA）官能团。后者通过一个桥连金属离子选择性地结合多组氨酸序列。NTA-DCF共轭物自身及其镍（II）配合物都具有与母体DCF荧光团相似的光物理性质。发射对顺磁性离子不敏感，这使得该探针能够在HEK 293-T和HeLa细胞的细胞外表面上对His6标签蛋白进行荧光标记。
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