Wang Dong-Mei, Liu Jing-Han, Zhou Jun, Ouyang Xi-Lin, Xing Yan-Chao
Department of Blood Transfusion, Urumqi General Hospital, Lanzhou Military Area, Urumqi 830000, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Dec;13(6):1094-8.
This study was aimed to investigate the inhibiting effects of adenosine on platelet in vitro in order to select functional protectants for platelets before lyophilization. Platelet membrane surface CD62P expression was assayed by flow cytometer (FCM). Platelet aggregations induced by restocetin, thrombin, ADP and propyl gallate were detected by APACT-2. The results showed that platelets membrane surface CD62P expression increased significantly after pre-treating of freeze-drying. 0.75 mmol/L adenosine could inhibit CD62P expression in a dose-dependent manner. Adenosine could inhibit platelet aggregation induced by propyl gallate, but no action on restocetin. When adenosine concentration was 1.0 mmol/L or higher, the aggregation induced by thrombin was significantly restrained. When concentration of adenosine was 0.75 mmol/L, platelet activation resulted from retreating could be inhibited and platelet aggregation induced by restocetin and thrombin were not affected markedly. It is concluded that adenosine can be one of the functional protectants and activation inhibitors in vitro for platelet cryo-preservation.
本研究旨在体外研究腺苷对血小板的抑制作用，以便在冻干前选择血小板的功能性保护剂。采用流式细胞仪（FCM）检测血小板膜表面CD62P的表达。用APACT-2检测瑞斯托霉素、凝血酶、ADP和没食子酸丙酯诱导的血小板聚集。结果表明，冻干预处理后血小板膜表面CD62P表达显著增加。0.75 mmol/L腺苷能以剂量依赖方式抑制CD62P表达。腺苷能抑制没食子酸丙酯诱导的血小板聚集，但对瑞斯托霉素无作用。当腺苷浓度为1.0 mmol/L及以上时，凝血酶诱导的聚集受到显著抑制。当腺苷浓度为0.75 mmol/L时，可抑制复温引起的血小板活化，对瑞斯托霉素和凝血酶诱导的血小板聚集无明显影响。结论：腺苷可作为血小板低温保存体外的功能性保护剂和活化抑制剂之一。
