Qin Fei, He Ji, Zhu Fa-Ming, Liu Jin-Hui, Chen Shu, Fu Qi-Hua, Yan Li-Xing
Blood Center of Zhejiang Province, Hangzhou 310006, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Dec;13(6):1117-9.
This study was aimed to establish one tube PCR reaction technique to determine ABO blood group genotypes. Salting-out method was adopted to extract genomic DNA; one tube polymerase chain reaction with GeneScan technique was used to identify ABO genotypes. The results showed that the ABO genotypes of 132 samples were in accordance with the phenotypes determined by serological technique. The frequencies of A, B and O were 0.205, 0.159 and 0.636 respectively. AA, AO, AB, BB, BO and OO genotypes were 8 (6.1%), 31 (23.5%), 7 (5.3%), 6 (4.5%), 23 (17.4%), and 57 (43.2%) respectively. It is concluded that one tube polymerase chain reaction with GeneScan technique can determine the genotypes of ABO blood group.
本研究旨在建立一种用于确定ABO血型基因型的单管PCR反应技术。采用盐析法提取基因组DNA；运用带有基因扫描技术的单管聚合酶链反应来鉴定ABO基因型。结果显示，132份样本的ABO基因型与血清学技术确定的表型相符。A、B和O的频率分别为0.205、0.159和0.636。AA、AO、AB、BB、BO和OO基因型分别为8例（6.1%）、31例（23.5%）、7例（5.3%）、6例（4.5%）、23例（17.4%）和57例（43.2%）。结论是，带有基因扫描技术的单管聚合酶链反应能够确定ABO血型的基因型。
