Czeczot Hanna, Scibior Dorota, Skrzycki Michał, Podsiad Małgorzata
Chair and Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland.
Acta Biochim Pol. 2006;53(1):237-42. Epub 2006 Jan 9.
We investigated glutathione level, activities of selenium independent GSH peroxidase, selenium dependent GSH peroxidase, GSH S-transferase, GSH reductase and the rate of lipid peroxidation expressed as the level of malondialdehyde in liver tissues obtained from patients diagnosed with cirrhosis or hepatocellular carcinoma. GSH level was found to be lower in malignant tissues compared to adjacent normal tissues and it was higher in cancer than in cirrhotic tissue. Non-Se-GSH-Px activity was lower in cancer tissue compared with adjacent normal liver or cirrhotic tissue, while Se-GSH-Px activity in cancer was found to be similar to its activity in cirrhotic tissue and lower compared to control tissue. An increase in GST activity was observed in cirrhotic tissue compared with cancer tissue, whereas the GST activity in cancer was lower than in adjacent normal tissue. The activity of GSH-R was similar in cirrhotic and cancer tissues, but higher in cancer tissue compared to control liver tissue. An increased level of MDA was found in cancer tissue in comparison with control tissue, besides its level was higher in cancer tissue than in cirrhotic tissue. Our results show that the antioxidant system of cirrhosis and hepatocellular carcinoma is severely impaired. This is associated with changes of glutathione level and activities of GSH-dependent enzymes in liver tissue. GSH and enzymes cooperating with it are important factors in the process of liver diseases development.
我们研究了从被诊断为肝硬化或肝细胞癌的患者获取的肝组织中的谷胱甘肽水平、硒非依赖性谷胱甘肽过氧化物酶、硒依赖性谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶、谷胱甘肽还原酶的活性以及以丙二醛水平表示的脂质过氧化率。结果发现，与相邻正常组织相比，恶性组织中的谷胱甘肽水平较低，且癌症组织中的谷胱甘肽水平高于肝硬化组织。与相邻正常肝组织或肝硬化组织相比，癌组织中的非硒谷胱甘肽过氧化物酶活性较低；而癌组织中的硒谷胱甘肽过氧化物酶活性与其在肝硬化组织中的活性相似，但低于对照组织。与癌组织相比，肝硬化组织中的谷胱甘肽S-转移酶活性增加，而癌组织中的谷胱甘肽S-转移酶活性低于相邻正常组织。肝硬化组织和癌组织中的谷胱甘肽还原酶活性相似，但癌组织中的谷胱甘肽还原酶活性高于对照肝组织。与对照组织相比，癌组织中的丙二醛水平升高，此外，癌组织中的丙二醛水平高于肝硬化组织。我们的结果表明，肝硬化和肝细胞癌的抗氧化系统严重受损。这与肝组织中谷胱甘肽水平和谷胱甘肽依赖性酶活性的变化有关。谷胱甘肽及其协同作用的酶是肝脏疾病发展过程中的重要因素。
