Wang Tianchi, Ran Yidong, Atkinson Ross G, Gleave Andrew P, Cohen Dan
The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand.
Plant Cell Rep. 2006 May;25(5):425-31. doi: 10.1007/s00299-005-0080-7. Epub 2006 Jan 11.
Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (beta-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.
已制定了毛花猕猴桃的再生及农杆菌介导转化方案。毛花猕猴桃具有诸多特性，使其成为猕猴桃功能基因组学研究的有用工具：该藤蔓相对较小且生长不旺盛，花朵遍布藤蔓，包括下部腋生枝条，并且在温室条件下该物种大量开花结果。在可控温室中，转基因毛花猕猴桃植株在转化后2年内实现了开花结果。在叶片、茎、根、花瓣和果实组织中观察到了指示uidA基因稳定表达的GUS（β-葡萄糖醛酸酶）活性。通过PCR和DNA凝胶印迹分析获得了转基因整合到毛花猕猴桃基因组中的分子证据。在子代幼苗中证明了转基因表型的遗传。已利用转基因毛花猕猴桃植株开展了猕猴桃的功能基因组学研究。
