Akopian V P, Avetisian S A, Davtian T K
Eksp Klin Farmakol. 2005 Nov-Dec;68(6):36-9.
The influence of colchicine on the spontaneous and chemotaxis-, protein kinase C-, and phagocytosis-induced respiratory burst of neutrophils and monocytes in the peripheral blood of patients with familial Mediterranean fever (FMF) has been studied. The transient activation of neutrophils and monocytes on the level of a single cell has been monitored by means of flow cytofluorimetry. It is shown that colchicine blocks the induction of chemotaxis-, phagocytosis-, and proteinkinase C-dependent respiratory burst in vitro, as well as the increased pro-oxidant transient activation of neutrophils and monocytes of FMF patients, both in the period of remission and during the FMF attack. Colchicine stimulates the intensity of the spontaneous respiratory burst of neutrophils and monocytes in patients in the course of remission and during the FMF attack. At the same time, the drug effectively suppresses the periodicity of the multidirectional transient activation of the respiratory burst of effector cells during FMF.
研究了秋水仙碱对家族性地中海热（FMF）患者外周血中性粒细胞和单核细胞的自发呼吸爆发以及趋化作用、蛋白激酶C和吞噬作用诱导的呼吸爆发的影响。通过流式细胞荧光测定法监测了单个细胞水平上中性粒细胞和单核细胞的瞬时激活。结果表明，秋水仙碱在体外可阻断趋化作用、吞噬作用和蛋白激酶C依赖性呼吸爆发的诱导，以及FMF患者中性粒细胞和单核细胞在缓解期和FMF发作期间促氧化剂瞬时激活的增加。秋水仙碱可刺激缓解期患者和FMF发作期间患者中性粒细胞和单核细胞自发呼吸爆发的强度。同时，该药物有效抑制了FMF期间效应细胞呼吸爆发多向瞬时激活的周期性。
