Pasquali Vittorio, Renzi Paolo
Dipartimento di Psicologia, Università di Roma La Sapienza, Rome, Italy.
Behav Res Methods. 2005 Aug;37(3):522-7. doi: 10.3758/bf03192723.
Modified motion detectors can be used to monitor locomotor activity and measure endogenous rhythms. Although these devices can help monitor insects in their home cages, the small size of the animals requires a very short wavelength detector. We modified a commercial microwave-based detection device, connected the detector's output to the digital input of a computer, and validated the device by recording circadian and ultradian rhythms. Periplaneta americana were housed in individual cages, and their activity was monitored at 18 degrees C and subsequently at 28 degrees C in constant darkness. Time series were analyzed by a discrete Fourier transform and a chi-square periodogram. Q10 values and the circadian free-running period confirmed the data reported in the literature, validating the apparatus. Moreover, the spectral analysis and periodogram revealed the presence of ultradian rhythmicity in the range of 1-8 h.
改良后的运动探测器可用于监测运动活动并测量内源性节律。尽管这些设备有助于在家笼中监测昆虫,但动物体型小需要非常短波长的探测器。我们对一种基于微波的商用检测设备进行了改良,将探测器的输出连接到计算机的数字输入,并通过记录昼夜节律和超日节律来验证该设备。将美洲大蠊饲养在单独的笼子里,在18摄氏度下监测它们的活动,随后在28摄氏度的持续黑暗环境中进行监测。通过离散傅里叶变换和卡方周期图对时间序列进行分析。Q10值和昼夜自由运行周期证实了文献中报道的数据,验证了该仪器。此外,频谱分析和周期图揭示了1 - 8小时范围内超日节律的存在。