Parveen S, Lukasik J, Scott T M, Tamplin M L, Portier K M, Sheperd S, Braun K, Farrah S R
Food Science and Technology Program, University of Maryland Eastern Shore, Princess Anne, MD 21853, USA.
J Appl Microbiol. 2006;100(1):50-7. doi: 10.1111/j.1365-2672.2005.02773.x.
The aim of this study was to assess geographical variation in multiple antibiotic resistance (MAR) profiles of livestock Escherichia coli as well as to evaluate the ability of MAR profiles to differentiate sources of faecal pollution.
More than 2000 E. coli isolates were collected from water retention ponds and manure of swine, poultry, beef and dairy farms in south, central and north Florida, and analysed for MAR using nine antibiotics. There were significant differences in antibiotic resistance of E. coli by season and livestock type for more than one antibiotic, but regional differences were significant only for ampicillin. Over the three regions, discriminant analysis using MAR profiles correctly classified 27% of swine, 49% of poultry, 56% of beef and 51% of dairy isolates.
Regional variations in MAR combined with moderate discrimination success suggest that MAR profiles of E. coli may only be marginally successful in identifying sources of faecal pollution.
This study demonstrates the existence of regional and seasonal differences in MAR profiles as well as the limited ability of MAR profiles to discriminate among livestock sources.
本研究旨在评估家畜大肠杆菌多重抗生素耐药性（MAR）谱的地理差异，并评估MAR谱区分粪便污染来源的能力。
从佛罗里达州南部、中部和北部的猪、家禽、肉牛和奶牛场的蓄水池和粪便中收集了2000多株大肠杆菌分离株，并用9种抗生素分析其MAR情况。超过一种抗生素的大肠杆菌耐药性在季节和家畜类型方面存在显著差异，但仅氨苄西林存在区域差异。在三个地区，使用MAR谱进行判别分析正确分类了27%的猪分离株、49%的家禽分离株、56%的肉牛分离株和51%的奶牛分离株。
MAR的区域差异以及中等程度的判别成功率表明，大肠杆菌的MAR谱在识别粪便污染来源方面可能仅取得了有限的成功。
本研究证明了MAR谱存在区域和季节差异，以及MAR谱区分家畜来源的能力有限。
