Grimes Adrian C, Stadt Harriett A, Shepherd Iain T, Kirby Margaret L
Medical University of South Carolina, Molecular and Cellular Biology and Pathobiology, 173 Ashley Avenue, Charleston, SC 29425, USA.
Dev Biol. 2006 Feb 15;290(2):265-76. doi: 10.1016/j.ydbio.2005.11.042. Epub 2006 Jan 10.
It is a widely held belief that the arterial pole of the zebrafish heart is unusual among models of comparative cardiogenesis. This is based, in part, on the report that the bulbus arteriosus undergoes a striated-to-smooth muscle phenotypic transition during development. An implication of this is that the zebrafish, a model almost ubiquitously accepted in other fields of comparative biology, may be poorly suited to the study of conotruncal abnormalities in human disease. However, while the use of atrioventricular-specific molecular markers has allowed extensive characterization of the development of the atrium and ventricle, the lack of any bulbus-specific markers has meant that this region of the zebrafish heart is poorly characterized and quite possibly misunderstood. We have discovered that the fluorescent nitric oxide indicator 4,5-diaminofluorescein diacetate (DAF-2DA) specifically labels the bulbus arteriosus throughout development from approximately 48 h post-fertilization. Therefore, using DAF-2DA and an immunohistochemical approach, we attempted to further characterize the development of the bulbus. We have concluded that no such phenotypic transition occurs, that contrary to current thinking, aspects of zebrafish arterial pole development are evolutionarily conserved, and that the bulbus should not be considered a chamber, being more akin to the arterial trunk(s) of higher vertebrates.
人们普遍认为，斑马鱼心脏的动脉极在比较心脏发生模型中是不同寻常的。这部分是基于这样的报告：动脉球在发育过程中经历了从横纹肌到平滑肌的表型转变。这意味着斑马鱼，这个在比较生物学的其他领域几乎被普遍接受的模型，可能不太适合用于研究人类疾病中的圆锥动脉干异常。然而，虽然使用房室特异性分子标记已经对心房和心室的发育进行了广泛的表征，但缺乏任何动脉球特异性标记意味着斑马鱼心脏的这个区域表征不足，很可能被误解。我们发现，荧光一氧化氮指示剂4,5-二氨基荧光素二乙酸酯（DAF-2DA）在受精后约48小时后的整个发育过程中特异性标记动脉球。因此，使用DAF-2DA和免疫组织化学方法，我们试图进一步表征动脉球的发育。我们得出的结论是，不存在这样的表型转变，与当前的观点相反，斑马鱼动脉极发育的某些方面在进化上是保守的，并且动脉球不应被视为一个腔室，它更类似于高等脊椎动物的动脉干。
