电穿孔作为一种将质粒pRL489导入颤藻MKU 277的工具。
Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277.
作者信息
Ravindran C R Marutha, Suguna S, Shanmugasundaram S
机构信息
Department of Pharmacology, M.C. 7764, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
出版信息
J Microbiol Methods. 2006 Jul;66(1):174-6. doi: 10.1016/j.mimet.2005.11.011. Epub 2006 Jan 9.
PMID:16406130
Abstract
To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/mug of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering.
摘要
为了在蓝细菌中建立基因转移系统，我们通过电穿孔成功地将穿梭质粒pRL489导入颤藻属MKU 277，转化频率高达每微克质粒DNA 102个菌落形成单位。这些发现表明，颤藻属MKU 277可作为基因操作的实验工具。
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