Morita Tomotake, Habe Hiroshi, Fukuoka Tokuma, Imura Tomohiro, Kitamoto Dai
Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5-2, 1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
J Biosci Bioeng. 2007 Dec;104(6):517-20. doi: 10.1263/jbb.104.517.
A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/mug of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.
一种通过电穿孔进行转化的便捷方法针对假丝酵母属进行了优化。使用赋予潮霉素B抗性的质粒pUXV1成功实现了转化；最大转化效率为每微克质粒DNA产生48个转化子。通过该方法获得了表达绿色荧光蛋白的南极假丝酵母T-34转化子。
