Takahashi Shuichiro, Komatsu Ken, Kagiwada Satoshi, Ozeki Johji, Mori Takuma, Hirata Hisae, Yamaji Yasuyuki, Ugaki Masashi, Namba Shigetou
Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
Virus Res. 2006 Mar;116(1-2):214-7. doi: 10.1016/j.virusres.2005.11.002. Epub 2006 Jan 10.
RNA silencing is a natural defense response against viral infection. This phenomenon has been used to interfere with viral infections by exploiting fragments of viral genomes as sources of RNA silencing. Agrobacterium-mediated transient expression of a hairpin RNA derived from the TGBp1 gene of Potato virus X (PVX) induced RNA silencing of the TGBp1 gene and resulted in interference of PVX infection. The interference was induced in the infiltrated leaves but not in the upper non-infiltrated leaves. Transient expression of a CP hairpin RNA also induced interference of PVX. The TGBp1 hairpin RNA showed more efficient interference of PVX infection than the CP hairpin RNA.
RNA沉默是针对病毒感染的一种天然防御反应。这种现象已被用于通过利用病毒基因组片段作为RNA沉默的来源来干扰病毒感染。农杆菌介导的源自马铃薯X病毒（PVX）TGBp1基因的发夹RNA的瞬时表达诱导了TGBp1基因的RNA沉默，并导致PVX感染受到干扰。这种干扰在浸润叶片中诱导产生，但在上部未浸润叶片中未出现。CP发夹RNA的瞬时表达也诱导了对PVX的干扰。与CP发夹RNA相比，TGBp1发夹RNA对PVX感染的干扰更有效。
