Obata Toshio
Department of Analytical Chemistry, Ohu University School of Pharmaceutical Sciences, Koriyama, Fukushima 963-8611, Japan.
Neurosci Lett. 2006 May 1;398(1-2):50-2. doi: 10.1016/j.neulet.2005.12.040. Epub 2006 Jan 6.
The present study examined the effect of chelerlythrine, a protein kinase C (PKC) inhibitor, on 1-methyl-4-phenylpyridine (MPP+)-induced hydroxyl radicals (*OH) in rat striatum. Rats were anesthetized, and sodium salicylate (0.5 mM or 0.5 nmol/microl/min) was infused through a microdialysis probe to detect the *OH generation as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (2,3-DHBA) in the striatum. Dopamine (DA)-selective neurotoxin, MPP+, infusion into the striatum of rats induces *OH formation, trapped as 2,3-DHBA. The application of chelerythrine, a potent and selective protein kinase C (PKC) inhibitor, suppressed MPP+ -induced *OH formation. The results in the present study suggests the protective effect of chelerythrine on *OH generation induced by MPP+.
本研究考察了蛋白激酶C（PKC）抑制剂白屈菜红碱对1-甲基-4-苯基吡啶（MPP+）诱导的大鼠纹状体羟自由基（OH）的影响。将大鼠麻醉后，通过微透析探针注入水杨酸钠（0.5 mM或0.5 nmol/微升/分钟），以检测纹状体中2,3-二羟基苯甲酸（2,3-DHBA）的非酶促形成所反映的OH生成。向大鼠纹状体中注入多巴胺（DA）选择性神经毒素MPP+会诱导OH形成，并以2,3-DHBA的形式捕获。强效选择性蛋白激酶C（PKC）抑制剂白屈菜红碱的应用可抑制MPP+诱导的OH形成。本研究结果表明白屈菜红碱对MPP+诱导的*OH生成具有保护作用。
