Cutroneo P, Beljean M, Luu R Phan Tan, Siouffi A-M
Universita degli Studi, Messina, Italy.
J Pharm Biomed Anal. 2006 May 3;41(2):333-40. doi: 10.1016/j.jpba.2005.10.050. Epub 2006 Jan 6.
A chemometric procedure is described to optimize the separation of some drugs used in the treatment of psychotic disorders: haloperidol, levomepromazine, risperidone, venlafaxine, carbamazepine and their main metabolites: reduced haloperidol, 9-hydroxy risperidone, desmethyl levomepromazine, desmethyl venlafaxine. The purpose of the procedure is the unambiguous identification and detection in biological fluids. Isocratic reversed-phase liquid chromatography with diode array detection was utilized. An experimental design methodology was carried out in which the experimental response is selectivity. In this way the designs for mixture compounds and for process variables (five variables) was performed which produced 36 experiments to carry out. The desirability function was used to select optimum separation conditions. The procedure provides a chromatogram of well separated solutes.
描述了一种化学计量学方法，用于优化治疗精神障碍所用的某些药物的分离：氟哌啶醇、左美丙嗪、利培酮、文拉法辛、卡马西平及其主要代谢物：还原氟哌啶醇、9-羟基利培酮、去甲基左美丙嗪、去甲基文拉法辛。该方法的目的是在生物流体中进行明确的鉴定和检测。采用了具有二极管阵列检测的等度反相液相色谱法。进行了一种实验设计方法，其中实验响应为选择性。通过这种方式，对混合化合物和过程变量（五个变量）进行了设计，产生了36个实验要进行。使用合意性函数来选择最佳分离条件。该方法提供了溶质分离良好的色谱图。
