Wu Sen, Wu Yuanyuan, Capecchi Mario R
Interdepartmental Program in Neuroscience, University of Utah, Salt Lake City, Utah 84112, USA.
Development. 2006 Feb;133(4):581-90. doi: 10.1242/dev.02236. Epub 2006 Jan 11.
Olig gene expression is proposed to mark the common progenitors of motoneurons and oligodendrocytes. In an attempt to further dissect the in vivo lineage relationships between motoneurons and oligodendrocytes, we used a conditional cell-ablation approach to kill Olig-expressing cells. Although differentiated motoneurons and oligodendrocytes were eliminated, our ablation study revealed a continuous generation and subsequent death of their precursors. Most remarkably, a normal number of oligodendrocyte precursors are formed at day 12 of mouse development, after all motoneuron precursors have been killed. The data presented herein supports a sequential model in which motoneuron and oligodendrocyte precursors are sequentially generated in vivo from neuroepithelial stem cells, but do not share a common lineage-restricted progenitor.
少突胶质细胞转录因子（Olig）基因表达被认为是运动神经元和少突胶质细胞共同祖细胞的标志。为了进一步剖析运动神经元和少突胶质细胞在体内的谱系关系，我们采用了条件性细胞消融方法来杀死表达Olig的细胞。尽管分化的运动神经元和少突胶质细胞被消除了，但我们的消融研究揭示了它们前体细胞的持续产生和随后的死亡。最值得注意的是，在小鼠发育第12天，在所有运动神经元前体细胞被杀死后，仍形成了正常数量的少突胶质细胞前体。本文提供的数据支持一种顺序模型，即运动神经元和少突胶质细胞前体在体内由神经上皮干细胞依次产生，但并不共享一个共同的谱系受限祖细胞。
