Nambiar P R, Kirchain S M, Courmier K, Xu S, Taylor N S, Theve E J, Patterson M M, Fox J G
Prashant R Nambiar, Division of Comparative Medicine, Massachusetts Institute of Technology, Building 16-849, Cambridge, MA 02139, USA.
Vet Pathol. 2006 Jan;43(1):2-14. doi: 10.1354/vp.43-1-2.
Helicobacter spp. have been implicated in a variety of gastrointestinal tract diseases, including peptic ulcer disease, gastric cancer, and inflammatory bowel disease (IBD), in humans and animals. Although most models of IBD are experimentally induced, spontaneous or natural models of IBD are rare. Herein, we describe a long-term study of chronic, progressive lesions that develop in the distal portion of the large bowel of unmanipulated Syrian hamsters naturally infected with Helicobacter spp. Twenty-four Syrian hamsters of three age groups (group A, 1 month [n = 4], group B, 7-12 months [n = 12], group C, 18-24 months [n = 12]), underwent complete postmortem examination. Results of microbial isolation and polymerase chain reaction and restriction fragment length polymorphism analyses confirmed the presence of Helicobacter spp. infection in the distal portion of the large bowel of all animals. Additionally, confounding pathogens, such as Clostridium difficile, Lawsonia intracellularis, and Giardia spp. that can cause proliferative enteritis, were absent in the hamsters of this study. Histopathologic scores for inflammation (P < 0.01), hyperplasia (P < 0.01), and dysplasia (P < 0.05) were significantly higher in the ileocecocolic (ICC) junction of animals in group C, relative to group A. Dysplastic lesions of various grades were detected in 5 of 11 hamsters in group C. Interestingly, the segment of the bowel that is usually colonized by Helicobacter spp. in hamsters had the most severe lesions. One hamster of group C developed a malignant fibrous histiocytoma, whereas another hamster developed a round cell sarcoma originating from the ICC junction. Thus, lesions in the distal portion of the large bowel of aging hamsters naturally colonized with Helicobacter spp. warrants developing the hamster as an animal model of IBD and potentially IBD-related cancer.
幽门螺杆菌属与人类和动物的多种胃肠道疾病有关，包括消化性溃疡病、胃癌和炎症性肠病（IBD）。虽然大多数IBD模型是通过实验诱导的，但IBD的自发或自然模型很少见。在此，我们描述了一项对自然感染幽门螺杆菌属的未处理叙利亚仓鼠大肠远端发生的慢性、进行性病变的长期研究。24只三个年龄组的叙利亚仓鼠（A组，1个月龄[n = 4]；B组，7 - 12个月龄[n = 12]；C组，18 - 24个月龄[n = 12]）接受了完整的尸检。微生物分离以及聚合酶链反应和限制性片段长度多态性分析结果证实所有动物的大肠远端均存在幽门螺杆菌属感染。此外，本研究的仓鼠中不存在可导致增生性肠炎的混杂病原体，如艰难梭菌、胞内劳森菌和贾第虫属。与A组相比，C组动物回盲结肠（ICC）交界处的炎症（P < 0.01）、增生（P < 0.01）和发育异常（P < 0.05）的组织病理学评分显著更高。C组11只仓鼠中有5只检测到不同程度的发育异常病变。有趣的是，仓鼠中通常被幽门螺杆菌属定殖的肠段病变最严重。C组一只仓鼠发生了恶性纤维组织细胞瘤，而另一只仓鼠发生了起源于ICC交界处的圆形细胞肉瘤。因此，自然定殖有幽门螺杆菌属的老龄仓鼠大肠远端的病变值得将仓鼠开发为IBD以及潜在的IBD相关癌症的动物模型。
