Pavlikhina L V, Eliseeva Iu E, Pozdnev V F, Orekhovich V N
Vopr Med Khim. 1975 Jan-Feb;21(1):54-60.
Based on fluorometric determination of a dipeptide histidil-leucine, which is splitted off from a synthetic substrate (Cbz-Phe-His-Leu) by carboxycathepsin (carboxydipeptidyl peptide hydrolase), a method was developed for estimation of the enzymatic activity in human blood serum. The method is characterized by simplicity and high sensitivity; Use of small amount of blood serum (about 0.03 ml) was possible. In normal human blood serum the carboxycathepsin activity varied from 7.5 to 18 nmoles of the dipeptide, liberated per mg of protein per hr.
基于对二肽组氨酰 - 亮氨酸的荧光测定，该二肽由羧基组织蛋白酶（羧基二肽基肽水解酶）从合成底物（苄氧羰基 - 苯丙氨酸 - 组氨酸 - 亮氨酸）上裂解下来，由此开发出一种用于估算人血清中酶活性的方法。该方法具有操作简单和灵敏度高的特点；使用少量血清（约0.03毫升）即可。在正常人血清中，羧基组织蛋白酶的活性为每毫克蛋白质每小时释放7.5至18纳摩尔的二肽。
