Feng Min, Li Pei
School of Pharmaceutical Sciences, Sun Yet-sen University, Guangzhou 510089, China.
Yao Xue Xue Bao. 2005 Oct;40(10):893-7.
To investigate the properties of cationic nanoparticles composed of poly (ethyleneimine)-g-poly (methyl methacrylate) (PEI-PMMA) as gene delivery carriers and explore the mechanism of PEI-PMMA nanoparticles mediated gene transfer.
PEI-PMMA nanoparticles were synthesized by free radical polymerization. The morphology of nanoparticles was observed by scanning electron microscopy. The particle size and zeta potential were measured by zeta sizer. The complex between pGL3 plasmid and nanoparticles was analyzed by gel electrophoresis; and PEI-PMMA nanoparticles mediated gene transfer into HeLa cells was observed by confocal laser scanning microscopy.
PEI-PMMA nanoparticles are spherical shape and monodispersity. The particle size and zeta potential are 172 nm and +50.3 mV, respectively. When pGL3 plasmid complexed with nanoparticles at N/P ratio of 5: 1 and 20: 1, the particle size of pGL3/nanoparticle complex are 133 and 139 nm and zeta potential is + 21.4 and + 33.7 mV, respectively. pGL3 plasmid complexed with nanoparticles completely at N/P ratio of 5: 1. PEI-PMMA nanoparticles can deliver pGL3 plasmid into HeLa cells by endocytosis and release pGL3 into the cytosol.
PEI-PMMA nanoparticles effectively transferred DNA to target cells and it is a promising non-viral carrier for gene delivery.
研究聚（乙烯亚胺）-g-聚（甲基丙烯酸甲酯）（PEI-PMMA）组成的阳离子纳米颗粒作为基因递送载体的性质，并探讨PEI-PMMA纳米颗粒介导基因转移的机制。
通过自由基聚合合成PEI-PMMA纳米颗粒。用扫描电子显微镜观察纳米颗粒的形态。用zeta粒度仪测量粒径和zeta电位。通过凝胶电泳分析pGL3质粒与纳米颗粒之间的复合物；并用共聚焦激光扫描显微镜观察PEI-PMMA纳米颗粒介导的基因向HeLa细胞的转移。
PEI-PMMA纳米颗粒呈球形且具有单分散性。粒径和zeta电位分别为172 nm和+50.3 mV。当pGL3质粒与纳米颗粒以N/P比为5:1和20:1复合时，pGL3/纳米颗粒复合物的粒径分别为133和139 nm，zeta电位分别为+21.4和+33.7 mV。pGL3质粒在N/P比为5:1时与纳米颗粒完全复合。PEI-PMMA纳米颗粒可通过内吞作用将pGL3质粒递送至HeLa细胞，并将pGL3释放到细胞质中。
PEI-PMMA纳米颗粒能有效地将DNA转移至靶细胞，是一种有前景的非病毒基因递送载体。
