Zheng Wen-jie, Tang Fu-lin, Zhao Yan, Chen Hua, Dong Yi
Department of Rheumatology, Peking Union Medical College Hospital, Beijing 100730, China.
Zhonghua Yi Xue Za Zhi. 2005 Dec 7;85(46):3272-6.
To investigate the prevalence of antiepithelial cell antibody (AECA) in systemic vasculitis (SV) and the target antigen thereof.
Sera of 113 patients with SV of different kinds, 46 patients with Behcet's disease, 23 patients with Takayasu arteritis, 19 patients with Wegener's granulomatosis, 8 patients with polyarteritis nodosa, 7 patients with microscopic polyangiitis, and 10 patients with Churg-Strauss syndrome were collected to detect the protein expression of AECA by Western blotting, with the protein of the endothelial cells of the line EA. hy926 line as substrate. Two-dimensional electrophoresis combined with immunoblotting, liquid chromatography-electrospray ionization mass spectrography was used to detect the target antigens related to vasculitis. Sera of 57 patients with systemic lupus erythematosus (SLE), 25 patients with rheumatoid arthritis (RA), and 20 healthy persons were collected as controls.
The AECA-positive rate of the SV patients was 69.0%, not significantly different from that of the SLE patients (66.7%), but significantly higher than those of the RA patients (6.7%, P < 0.01) and healthy persons (0, P < 0.01). The AECA from the SV patients reacted with the endothelial cell (EC) antigens with the molecular size of 26 to 125 kDa, and the AECA from the SLE patients reacted with the EC antigens with the molecular size of 15 to 97 kDa. The EC antigens with the molecular size of 47 kDa was commonly found in the sera of the AECA-positive SV patients and SLE patients, however, was not found in the RA, and polymyositis-dermatomyositis patients. The EC protein reacted by 47 kDa protein was identified by proteomic techniques as alpha-enolase.
A group of heterogeneous antibodies, AECA can be found frequently in patients with SV and SLA. AECA reacts against a common EC antigen, alpha-enolase.
探讨抗上皮细胞抗体（AECA）在系统性血管炎（SV）中的患病率及其靶抗原。
收集113例不同类型SV患者、46例白塞病患者、23例大动脉炎患者、19例韦格纳肉芽肿患者、8例结节性多动脉炎患者、7例显微镜下多血管炎患者和10例变应性肉芽肿性血管炎患者的血清，以EA.hy926细胞系的内皮细胞蛋白为底物，采用蛋白质印迹法检测AECA的蛋白表达。采用二维电泳结合免疫印迹、液相色谱-电喷雾电离质谱法检测与血管炎相关的靶抗原。收集57例系统性红斑狼疮（SLE）患者、25例类风湿关节炎（RA）患者和20例健康人的血清作为对照。
SV患者的AECA阳性率为69.0%，与SLE患者（66.7%）无显著差异，但显著高于RA患者（6.7%，P<0.01）和健康人（0，P<0.01）。SV患者的AECA与分子大小为26至125 kDa的内皮细胞（EC）抗原反应，SLE患者的AECA与分子大小为15至97 kDa的EC抗原反应。分子大小为47 kDa的EC抗原常见于AECA阳性的SV患者和SLE患者血清中，而在RA和多发性肌炎-皮肌炎患者中未发现。通过蛋白质组学技术鉴定，与47 kDa蛋白反应的EC蛋白为α-烯醇化酶。
AECA是一组异质性抗体，在SV和SLA患者中常见。AECA与一种常见的EC抗原α-烯醇化酶反应。
