Zeng Xue-jun, Zhu Wei-guo, Deng Xue-xin, Tang Fu-lin, Dong Yi
Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Yi Xue Za Zhi. 2004 Oct 2;84(19):1629-32.
To investigate the prevalence of anti-endothelial cell antibodies (AECA) in systemic vasculitis and to assess the correlation between AECA and disease activity, and try to discuss the classification of AECA.
Cyto-ELISA with EA.hy926 and HMEC-1, two immortalized cell lines, as substrates, was applied to detect AECA in 122 cases of systemic vasculitis, including 43 cases of Behcet disease, 19 cases of Takayasu arteritis, 19 cases of Wegener's granulomatosis, 11 cases of microscopic polyangiitis, 9 cases of polyarteritis nodosa, 3 cases of Churg-Strause syndrome, 2 cases of giant cell arteritis and other 16 cases of which could not be classified clearly. Patients with SLE, RA, and fever of unknown origin, and normal persons were used as control groups. Then the associations of AECA to laboratory findings and clinical disease activity (scored by BVAS) were analyzed. Furthermore, the AECA value from the two different substrate cells were analyzed for correlation and difference.
With either EA.hy926 or HMEC-1 as substrates, the AECA prevalence rates of systemic vasculitis (respectively 33.61% and 37.70%) were significantly higher than those of the normal and RA groups (less than 10%), and the prevalence of AECA in SLE (61.75%) was higher than that of systemic vasculitis (37.70%) when using HMEC-1 as substrates. AECA was found to correlate very well with ESR in 122 cases of systemic vasculitis and with BVAS in 40 cases of small systemic vasuculitides, including Wegener's disease, microscopic polyangiitis, Churg-Strause syndrome and so on. The pair AECA values with EA.hy926 and HMEC-1 as substrate cells were found to be correlated significantly, and the matching rate was 92.62%.
Prevalence of AECA in systemic vasculitis is high. AECA indicates the clinical disease activity. As to the assumption of classification of AECA into antibodies against microvascular and macrovascular endothelial cells, further study need to be done to prove or disprove it.
研究抗内皮细胞抗体（AECA）在系统性血管炎中的患病率，评估AECA与疾病活动度之间的相关性，并尝试探讨AECA的分类。
采用以两种永生化细胞系EA.hy926和HMEC-1为底物的细胞酶联免疫吸附测定法（Cyto-ELISA），检测122例系统性血管炎患者血清中的AECA，其中包括43例白塞病、19例大动脉炎、19例韦格纳肉芽肿、11例显微镜下多血管炎、9例结节性多动脉炎、3例变应性肉芽肿性血管炎、2例巨细胞动脉炎以及其他16例无法明确分类的患者。以系统性红斑狼疮（SLE）、类风湿关节炎（RA）患者及不明原因发热患者和正常人为对照组。分析AECA与实验室检查结果及临床疾病活动度（采用BVAS评分）之间的相关性。此外，分析两种不同底物细胞的AECA值的相关性及差异。
以EA.hy926或HMEC-1为底物时，系统性血管炎患者的AECA患病率（分别为33.61%和37.70%）显著高于正常人和RA组（低于10%），以HMEC-1为底物时，SLE患者的AECA患病率（61.75%）高于系统性血管炎患者（37.70%）。在122例系统性血管炎患者中发现AECA与血沉（ESR）密切相关，在40例小血管炎（包括韦格纳病、显微镜下多血管炎、变应性肉芽肿性血管炎等）患者中AECA与BVAS密切相关。以EA.hy926和HMEC-1为底物细胞的AECA值显著相关，匹配率为92.62%。
系统性血管炎患者中AECA患病率较高。AECA提示临床疾病活动度。关于将AECA分为抗微血管和抗大血管内皮细胞抗体的假设，需进一步研究证实或证伪。
