Pei Xian-Wu, Wang Kun-Zheng, Dang Xiao-Qian, Song Jin-Hui, Shi Zhi-Bin, Gao Deng-Feng
Department of Orthopedics, Second Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710004, China.
Zhong Xi Yi Jie He Xue Bao. 2006 Jan;4(1):52-5. doi: 10.3736/jcim20060114.
To observe the promoting effect of arnebia root oils on expression of basic fibroblast growth factor (bFGF) in skin wound of rabbits and the histomorphological changes in the wound surface, and to discuss its mechanism.
Bilateral round skin wounds were made on the back of 15 rabbits. The three wounds on one side of the back of each rabbit were treated with arnebia root oils, while the three wounds on the other side were treated with vaseline in order to promote the wound healing. The histomorphology and ultrastructure under electron microscopy of the wounds, and the rate of wound healing were examined at different time. Western blotting assay was used to detect the expression of bFGF in the wound surface.
The healing rate of the arnebia root oils-treated wounds was evidently higher than that of the vaseline-treated wounds (P<0.05). The quantities of fibroblast, collagen and capillary in the arnebia root oils-treated wounds were much more than those in the vaseline-treated wounds, and the expression of endogenous bFGF in the arnebia root oils-treated wounds was enhanced obviously as compared with that in the vaseline-treated wounds in different period of wound healing. There existed a parallel correlation between the expression level of bFGF and the rate of wound healing.
The promoting effect of arnebia root oils on wound healing may be related to increasing the expression level of basic fibroblast growth factor in the skin wound.
观察紫草油对兔皮肤创面碱性成纤维细胞生长因子（bFGF）表达及创面组织形态学变化的促进作用，并探讨其作用机制。
将15只家兔背部制成双侧圆形皮肤创面。每只家兔背部一侧的3个创面用紫草油处理，另一侧的3个创面用凡士林处理，以促进创面愈合。在不同时间观察创面的组织形态学及电镜下超微结构，并检测创面愈合率。采用蛋白质免疫印迹法检测创面bFGF的表达。
紫草油处理组创面愈合率明显高于凡士林处理组（P<0.05）。紫草油处理组创面成纤维细胞、胶原及毛细血管数量均明显多于凡士林处理组，且在创面愈合不同时期，紫草油处理组创面内源性bFGF的表达明显增强。bFGF表达水平与创面愈合率呈平行相关。
紫草油促进创面愈合的作用可能与提高皮肤创面碱性成纤维细胞生长因子的表达水平有关。
