Wu Zhengzheng, Fan Lina, Xu Bin, Lin Yongliang, Zhang Peng, Wei Xing
1 Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China .
Tissue Eng Part A. 2015 Jan;21(1-2):390-402. doi: 10.1089/ten.TEA.2013.0260. Epub 2014 Oct 14.
Skin damage is one of the common clinical skin diseases, and the main cure is the use of skin graft, especially for large area of skin injury or deep-skin damage. However, skin graft demand is far greater than that currently available. In this study, xenogeneic decellularized scaffold was prepared with pig peritoneum by a series of biochemical treatments to retain normal three-dimensional tissue scaffold and remove cells and antigenic components from the tissue. Scaffold was combined with hyaluronic acid (HA) plus two different concentrations of basic fibroblast growth factor (bFGF) and tested for its use for the repair of skin wounds. HA enhanced bFGF to adsorb to the decellularized scaffolds and slowed the release of bFGF from the scaffolds in vitro. A total of 20 rabbits were sacrificed on day 3, 6, 11, or 14 postsurgery. The wound healing rate and the thickness of dermis layer of each wound were determined for analyzing the wound repair. Statistical analysis was performed by the two-tailed Student's t-test. Wounds covered with scaffolds containing 1 μg/mL bFGF had higher wound healing rates of 47.24%, 74.69%, and 87.54%, respectively, for days 6, 11, and 14 postsurgery than scaffolds alone with wound healing rates of 28.17%, 50.31%, and 61.36% and vaseline oil gauze with wound healing rates of 24.84%, 42.75%, and 57.62%. Wounds covered with scaffolds containing 1 μg/mL bFGF showed more dermis regeneration than the other wounds and had dermis layer of 210.60, 374.40, and 774.20 μm, respectively, for days 6, 11, and 14 postsurgery compared with scaffolds alone with dermis layer of 116.60, 200.00, and 455.40 μm and vaseline oil gauze with dermis layer of 82.60, 186.20, and 384.40 μm. There was no significant difference in wound healing rates and thickness of dermis layer between wounds covered with scaffolds containing 1 and 3 μg/mL bFGF on days 3, 6, 11, and 14 postsurgery. The decellularized scaffolds combined with HA and bFGF can be further tested for skin tissue engineering.
皮肤损伤是常见的临床皮肤病之一，主要的治疗方法是皮肤移植，尤其是对于大面积皮肤损伤或深度皮肤损伤。然而，皮肤移植的需求远远大于目前的供应量。在本研究中，通过一系列生化处理，用猪腹膜制备了异种脱细胞支架，以保留正常的三维组织支架，并从组织中去除细胞和抗原成分。将支架与透明质酸（HA）以及两种不同浓度的碱性成纤维细胞生长因子（bFGF）结合，并测试其用于修复皮肤伤口的效果。HA增强了bFGF对脱细胞支架的吸附，并在体外减缓了bFGF从支架中的释放。总共20只兔子在术后第3、6、11或14天被处死。测定每个伤口的愈合率和真皮层厚度以分析伤口修复情况。采用双侧Student t检验进行统计分析。术后第6、11和14天，覆盖含1μg/mL bFGF支架的伤口愈合率分别为47.24%、74.69%和87.54%，高于单独使用支架时的愈合率（分别为28.17%、50.31%和61.36%）以及凡士林油纱布时的愈合率（分别为24.84%、42.75%和57.62%）。覆盖含1μg/mL bFGF支架的伤口比其他伤口显示出更多的真皮再生，术后第6、11和14天的真皮层厚度分别为210.60、374.40和774.20μm，而单独使用支架时真皮层厚度分别为116.60、200.00和455.40μm，凡士林油纱布时真皮层厚度分别为82.60、186.20和384.40μm。术后第3、6、11和14天，覆盖含1μg/mL和3μg/mL bFGF支架的伤口在愈合率和真皮层厚度方面没有显著差异。脱细胞支架与HA和bFGF结合可进一步用于皮肤组织工程的测试。
