Augood S J, Emson P C
Department of Neuroendocrinology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Neuroscience. 1992;47(2):317-24. doi: 10.1016/0306-4522(92)90247-y.
The effect of a unilateral intrastriatal microinjection of pertussis toxin on the expression of proneurotensin and preproenkephalin A mRNAs in the adult rat neostriatum was investigated using a technique of non-radioactive in situ hybridization. Control sham microinjected animals received an equal volume of vehicle only and were processed in parallel with the pertussis toxin-treated rats. All rats were allowed to recover from the stereotaxic surgery for 22 h before being killed and their brains rapidly removed and processed for in situ hybridization using alkaline phosphatase-labelled oligonucleotide probes. In comparison to sham microinjected rats, a single intrastriatal microinjection of pertussis toxin (1 microgram) resulted in a significant increase in the amount of both proneurotensin and preproenkephalin A mRNAs in the ipsilateral neostriatum. For proneurotensin mRNA, this increase was reflected by a substantial increase in the number of mRNA-containing cells detected. Proneurotensin mRNA-containing cells detected in the nucleus accumbens appeared to be unaffected by the intrastriatal pertussis toxin microinjection. In contrast, the significant increase in preproenkephalin A mRNA, when compared to the contralateral uninjected striatum and the ipsilateral striatum of control sham injected rats, was reflected by an increase in the cellular amount of preproenkephalin A mRNA and not by an increase in the number of mRNA-containing cells detected. These results demonstrate that the expression of both proneurotensin mRNA and preproenkephalin A mRNA in the adult rat striatum are rapidly increased in vivo by an intrastriatal microinjection of pertussis toxin.
采用非放射性原位杂交技术，研究了成年大鼠新纹状体内单侧注射百日咳毒素对前神经降压素和前脑啡肽原A mRNA表达的影响。对照假注射动物仅接受等量的溶剂，并与百日咳毒素处理的大鼠并行处理。所有大鼠在立体定向手术后恢复22小时，然后处死，迅速取出大脑，使用碱性磷酸酶标记的寡核苷酸探针进行原位杂交处理。与假注射大鼠相比，单次纹状体内注射百日咳毒素（1微克）导致同侧纹状体内前神经降压素和前脑啡肽原A mRNA的量显著增加。对于前神经降压素mRNA，这种增加反映在检测到的含mRNA细胞数量的大幅增加上。伏隔核中检测到的含前神经降压素mRNA的细胞似乎不受纹状体内百日咳毒素注射的影响。相反，与对侧未注射的纹状体和对照假注射大鼠的同侧纹状体相比，前脑啡肽原A mRNA的显著增加反映在细胞内前脑啡肽原A mRNA量的增加上，而不是检测到的含mRNA细胞数量的增加上。这些结果表明，成年大鼠纹状体内注射百日咳毒素可在体内迅速增加前神经降压素mRNA和前脑啡肽原A mRNA的表达。
