Pejić Natasa, Kolar-Anić Ljiljana, Anić Slobodan, Stanisavljev Dragomir
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, YU-11000 Belgrade, Serbia and Montenegro.
J Pharm Biomed Anal. 2006 May 3;41(2):610-5. doi: 10.1016/j.jpba.2005.11.043. Epub 2006 Jan 18.
A new procedure for kinetic determination of paracetamol in pharmaceuticals is proposed. The method is based on potentiometric monitoring of the concentration perturbations of the matrix reaction system being in a stable non-equilibrium stationary state close to the bifurcation point. In the case considered as the matrix system, the Bray-Liebhafsky oscillatory reaction is used. The response of the matrix system to the perturbations by different concentrations of paracetamol is followed by a Pt-electrode. Proposed method relies on the linear relationship between maximal potential shift, DeltaEm, and the logarithm of added paracetamol amounts. It is obtained in optimized experimental conditions for variable amounts of paracetamol in the range 0.0085 and 1.5 micromol. The sensitivity and precision of proposed method were quite good (0.0027 micromol as the limit of detection and 2.4% as R.S.D.). Some aspects of possible chemical interactions between paracetamol and matrix are discussed. Applicability of the proposed method to the direct determination of paracetamol in pharmaceutical formulations was demonstrated.
提出了一种动力学测定药物中扑热息痛的新方法。该方法基于对处于接近分叉点的稳定非平衡稳态的基质反应体系浓度扰动的电位监测。在作为基质体系的情况下，使用布雷-利布哈夫斯基振荡反应。通过铂电极跟踪基质体系对不同浓度扑热息痛扰动的响应。所提出的方法依赖于最大电位偏移量ΔEm与添加扑热息痛量的对数之间的线性关系。这是在优化的实验条件下，针对0.0085至1.5微摩尔范围内不同量的扑热息痛获得的。所提出方法的灵敏度和精密度相当好（检测限为0.0027微摩尔，相对标准偏差为2.4%）。讨论了扑热息痛与基质之间可能的化学相互作用的一些方面。证明了所提出方法在直接测定药物制剂中扑热息痛方面的适用性。
