Schmidt Mark S, Huang Rong, Classon Robert J, Murry Daryl J
College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA.
J Pharm Biomed Anal. 2006 May 3;41(2):540-3. doi: 10.1016/j.jpba.2005.11.039. Epub 2006 Jan 18.
An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.
已开发并验证了一种使用大气压化学电离（APCI）的液相色谱-质谱（LC-MS）方法，以长春碱为内标物测定人血浆中的抗癌药物长春新碱。样品经固相萃取（SPE）后，定量下限（LLOQ）为0.18 ng/ml，检测下限为0.09 ng/ml，线性校准范围为0.18 - 180 ng/ml。作为一项药代动力学研究的一部分，该方法已用于测量29名婴儿静脉推注后0.08至24小时长春新碱的血浆浓度。24小时时长春新碱的浓度为0.2 - 1.36 ng/ml。
