Larsson M H, Rapp L, Lindström E
Department of Integrative Pharmacology, Gastrointestinal Biology, AstraZeneca R&D Mölndal, Mölndal, Sweden.
Neurogastroenterol Motil. 2006 Feb;18(2):144-52. doi: 10.1111/j.1365-2982.2005.00736.x.
The present study aimed at evaluating the effect of dextran sodium sulphate (DSS)-induced colitis on visceral sensitivity, measured as the visceromotor response (VMR) to colorectal distension (CRD) in BALB/c and C57Bl/6 male mice. Inflammation was induced by the addition of 4% DSS to the drinking water for 5 (C57Bl/6) or 6-7 days (BALB/c). Parallel groups were used to monitor histopathological changes and visceral sensitivity. Pseudo-affective visceral pain responses were evoked using an increasing phasic CRD paradigm (10-60 mmHg) in conscious mice on predetermined days (pretreatment controls, 12, 16, 20, 30, 40 and 51). In both mouse strains, significant histopathological changes developed between days 2 and 5 of DSS treatment, and persisted until day 12 (P < 0.05). On day 15, inflammatory scores were reduced by about 50%. Despite evidence of inflammation in DSS-treated mice, no differences could be shown in the VMR to CRD between DSS-treated mice and controls at any time point tested. In addition, no differences were seen before and after DSS treatment in the same group of mice. In conclusion, these data suggest that DSS-induced colonic inflammation does not affect the visceral sensitivity to CRD, neither at short or long term, in BALB/c or C57Bl/6 male mice.
本研究旨在评估葡聚糖硫酸钠（DSS）诱导的结肠炎对BALB/c和C57Bl/6雄性小鼠内脏敏感性的影响，以内脏运动反应（VMR）作为对结肠扩张（CRD）的测量指标。通过在饮用水中添加4% DSS持续5天（C57Bl/6）或6 - 7天（BALB/c）来诱导炎症。使用平行组监测组织病理学变化和内脏敏感性。在预定的天数（预处理对照组、第12、16、20、30、40和51天），对清醒小鼠采用递增的阶段性CRD范式（10 - 60 mmHg）诱发假性情感性内脏疼痛反应。在两种小鼠品系中，DSS治疗的第2天至第5天出现显著的组织病理学变化，并持续至第12天（P < 0.05）。在第15天，炎症评分降低了约50%。尽管有证据表明DSS处理的小鼠存在炎症，但在任何测试时间点，DSS处理的小鼠与对照组之间对CRD的VMR均无差异。此外，同一组小鼠在DSS处理前后也未见差异。总之，这些数据表明，DSS诱导的结肠炎症在短期或长期内均不影响BALB/c或C57Bl/6雄性小鼠对CRD的内脏敏感性。
