Silva A R, Cardoso R C S, Silva L D M
Laboratory of Carnivore Reproduction, State University of Ceará, Fortaleza, Brazil.
Reprod Domest Anim. 2006 Feb;41(1):74-8. doi: 10.1111/j.1439-0531.2006.00630.x.
Contents The aim of the present study was to compare the influence of room temperature (27 degrees C) and 4 degrees C during glycerol addition on canine semen cryopreservation and verify the effect of different post-thawing dilutions on canine semen. Ten ejaculates from five stud dogs were collected by digital manipulation. Semen samples were evaluated and further divided into two aliquots. The first aliquot was extended in Tris-egg yolk-glycerol at 27 degrees C and the second one received glycerol at 4 degrees C. Samples were frozen and stored in liquid nitrogen. After 1 week, samples were thawed and submitted to evaluations of progressive sperm motility, morphology, acrosomal integrity, hypo-osmotic swelling (HOST) and thermoresistance tests. For thermoresistance test, aliquots were divided in two portions: one portion was kept undiluted (1 : 0) and the other one was diluted in a 1 : 4 ratio (one part semen to four parts extender). No differences were observed between temperatures for glycerol addition regarding seminal parameters evaluated. Furthermore, post-thawing dilutions demonstrated similar effect on canine semen longevity. Correlations among post-thaw sperm motility and HOST and results from thermoresistance test were observed for both temperatures for glycerol addition. In conclusion, glycerol could be added to canine semen at room temperature (27 degrees C) or at 4 degrees C. Moreover, there is no need to extend canine semen after thawing for the thermoresistance test, but if we need to increase the inseminating volume for artificial inseminations, the addition of extender will not damage the semen.
内容 本研究的目的是比较甘油添加过程中室温（27摄氏度）和4摄氏度对犬精液冷冻保存的影响，并验证不同解冻后稀释液对犬精液的作用。通过手法采集了5只种公犬的10次射精。对精液样本进行评估，并进一步分为两份。第一份在27摄氏度下用Tris-蛋黄-甘油进行稀释，第二份在4摄氏度下添加甘油。样本冷冻后储存在液氮中。1周后，样本解冻并进行精子活力、形态、顶体完整性、低渗肿胀（HOST）和耐热性测试。对于耐热性测试，将样本分为两部分：一部分不稀释（1:0），另一部分以1:4的比例稀释（一份精液加四份稀释液）。在评估的精液参数方面，甘油添加温度之间未观察到差异。此外，解冻后稀释液对犬精液寿命显示出相似的作用。在甘油添加的两个温度下，均观察到解冻后精子活力与HOST之间以及耐热性测试结果之间存在相关性。总之，甘油可在室温（27摄氏度）或4摄氏度下添加到犬精液中。此外，进行耐热性测试时，解冻后无需对犬精液进行稀释，但如果我们需要增加人工授精的输精量，添加稀释液不会损害精液。
