Raimondo Stefania, Penna Claudia, Pagliaro Pasquale, Geuna Stefano
Department of Clinical and Biological Sciences, University of Turin, Orbassano, TO, Italy.
J Anat. 2006 Jan;208(1):3-12. doi: 10.1111/j.1469-7580.2006.00511.x.
Increasing attention is being given to the use of adult rather than embryonic stem cells, both for research and for the development of transplantation treatments for human disease. In particular, mesenchymal bone marrow stem cells have been studied extensively because of their ability to self-renew and to give rise to various differentiated cell types, and because of the relative ease with which they can be obtained and cultured. In addition, the possibility of labelling stem cells with green fluorescent protein before transplantation has opened new and promising perspectives for their use in basic research. Because no structural or ultrastructural description of adult mesenchymal stem cells is available in the literature, this paper describes their morphology as revealed by light, confocal and electron microscopy, focusing on cells that are particularly suitable for transplantation studies, i.e. those derived from rat bone marrow transfected with green fluorescent protein. The results provide a basis for experimental studies of the differentiation of these cells in normal and pathological tissues.
人们越来越关注使用成体干细胞而非胚胎干细胞，用于研究以及开发针对人类疾病的移植治疗方法。特别是间充质骨髓干细胞，由于其自我更新能力、分化产生各种细胞类型的能力，以及相对容易获取和培养的特点，受到了广泛研究。此外，在移植前用绿色荧光蛋白标记干细胞的可能性为其在基础研究中的应用开辟了新的、有前景的方向。由于文献中没有关于成体间充质干细胞的结构或超微结构描述，本文描述了通过光学显微镜、共聚焦显微镜和电子显微镜揭示的它们的形态，重点关注特别适合移植研究的细胞，即那些源自转染了绿色荧光蛋白的大鼠骨髓的细胞。这些结果为这些细胞在正常和病理组织中分化的实验研究提供了基础。
