Soleimanjahi Hoorieh, Roostaee Mohammad Hassan, Rasaee Mohammad Javad, Mahboudi Fereidoon, Kazemnejad Anooshirvan, Bamdad Taravat, Zandi Keivan
Department of Virology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
FEMS Immunol Med Microbiol. 2006 Feb;46(1):100-6. doi: 10.1111/j.1574-695X.2005.00015.x.
Herpes simplex virus produces primary and latent infections with periodic recurrency. The prime-boost immunization strategies were studied using a DNA vaccine carrying the full-length glycoprotein D-1 gene and a baculovirus-derived recombinant glycoprotein D, both expressing herpes simplex virus glycoprotein D-1 protein. Immunization with recombinant DNAs encoding antigenic proteins could induce cellular and humoral responses by providing antigen expression in vivo. Higher immune response, however, occurred when the recombinant proteins followed DNA inoculation. While all groups of the immunized mice and positive control group could resist virus challenge, a higher virus neutralizing antibody level was detected in the animals receiving recombinant protein following DNA vaccination.
单纯疱疹病毒会引发原发性感染和潜伏感染，并伴有周期性复发。使用携带全长糖蛋白D-1基因的DNA疫苗和杆状病毒衍生的重组糖蛋白D对初免-加强免疫策略进行了研究，二者均表达单纯疱疹病毒糖蛋白D-1蛋白。编码抗原蛋白的重组DNA免疫可通过在体内提供抗原表达来诱导细胞和体液免疫反应。然而，当重组蛋白在DNA接种后使用时，会产生更高的免疫反应。虽然所有免疫小鼠组和阳性对照组都能抵抗病毒攻击，但在DNA疫苗接种后接受重组蛋白的动物中检测到更高的病毒中和抗体水平。
