RNA干扰在体内对乙型肝炎病毒复制和表达的抑制作用

[Inhibition of hepatitis B virus replication and expression by RNA interference in vivo].

作者信息

Ying Ruo-su, Fan Xue-gong, Zhu Cai, Li Ning, Zhang Bao-xin

机构信息

Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2006 Jan;14(1):15-8.

PMID:16420758

Abstract

OBJECTIVE

To evaluate the inhibitory effect of small interfering RNA (siRNA) targeting HBV C gene region on hepatitis B virus (HBV) in vivo.

METHODS

An animal model of HBV infection was developed hydrodynamically, and pcDNA3.1-HBV and siRNA were together injected into the tail vein of the BALB/c mice. HBsAg was analyzed by time-resolved immunofluorometric assay, HBV DNA was analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR, and viral specific proteins (HBsAg and HBcAg) in the mice livers were assayed using immunohistochemical staining.

RESULTS

In the mice, the siRNA effectively inhibited HBV replication and expression compared with the controls. The inhibitive effect of siRNA on HBV lasted at least 3 days.

CONCLUSION

These results demonstrate that RNAi can substantially inhibit HBV replication and expression in vivo.

摘要

目的

评估靶向乙肝病毒（HBV）C基因区域的小干扰RNA（siRNA）在体内对乙肝病毒（HBV）的抑制作用。

方法

通过流体动力学方法建立HBV感染动物模型，将pcDNA3.1-HBV和siRNA一起注入BALB/c小鼠尾静脉。采用时间分辨免疫荧光分析法分析HBsAg，荧光定量PCR（FQ-PCR）法分析HBV DNA，半定量RT-PCR法检测HBV C-mRNA，免疫组织化学染色法检测小鼠肝脏中的病毒特异性蛋白（HBsAg和HBcAg）。