Wen Zhi-li, Tan De-ming, Peng Shi-fang, Hou Zhou-hua, Cheng Jun
Department of Gastroenterology, Nanchang First Hospital, Nanchang 330008, China.
Zhonghua Gan Zang Bing Za Zhi. 2006 Jan;14(1):19-22.
To study the relationship between quasispecies of hepatitis B virus and the clinical manifestations of their infection, and to find the answer of why different quasispecies of HBV with the same genotype can induce different clinical situations.
Sixty serum samples, in all of which HBVs of genotype B exist, taken from 32 chronic asymptomatic carriers and 28 severe chronic hepatitis patients, were collected to detect quasispecies of HBV DNA by melt curve approach. Then the relationship between quasispecies of HBV of the same genotype and the clinical situation of their infection was studied by comparing the wave crests of the two sample groups.
The data of the 60 serum samples of HBV of genotype B detected by melt curve showed that HBV DNA in severe chronic hepatitis patients had more wave crests than that in chronic asymptomatic carriers (P < 0.05), suggesting that HBV in severe chronic hepatitis patients had more quasispecies than in the chronic asymptomatic carriers.
The numbers of quasispecies of HBV correlate with the clinical situations of their infection. In the patients infected by HBV of the same genotype, those who have more HBV quasispecies would have more severe clinical manifestations.
研究乙型肝炎病毒准种与其感染临床表现之间的关系，并找出具有相同基因型的不同乙型肝炎病毒准种为何能引发不同临床情况的答案。
收集32例慢性无症状携带者和28例重度慢性肝炎患者的60份血清样本（所有样本中均存在B基因型乙型肝炎病毒），采用熔解曲线法检测乙型肝炎病毒DNA的准种。然后，通过比较两组样本的波峰，研究相同基因型乙型肝炎病毒准种与其感染临床情况之间的关系。
通过熔解曲线检测的60份B基因型乙型肝炎病毒血清样本数据显示，重度慢性肝炎患者的乙型肝炎病毒DNA波峰多于慢性无症状携带者（P < 0.05），这表明重度慢性肝炎患者的乙型肝炎病毒准种比慢性无症状携带者更多。
乙型肝炎病毒准种数量与其感染的临床情况相关。在感染相同基因型乙型肝炎病毒的患者中，乙型肝炎病毒准种较多的患者临床表现更为严重。
