• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

An investigation of the role of the copper in galactose oxidase.

作者信息

Cleveland L, Coffman R E, Coon P, Davis L

出版信息

Biochemistry. 1975 Mar 25;14(6):1108-15. doi: 10.1021/bi00677a003.

DOI:10.1021/bi00677a003
PMID:164209
Abstract

Galactose oxidase is a metalloenzyme containing a single copper atom per molecule. The mechanism of action of galactose oxidase is studied in this paper by investigating substrate specificity and activation by peroxidase, and probing the copper site by electron spin resonance (ESR) spectroscopy. Line-shape simulation of ESR spectra are also reported and a comparison is made between observed and simulated spectra for galactose oxidase. A comparison is also reported for the enzyme from various commercial sources and enzyme isolated from a fungus in this laboratory. The results of this investigation suggest that the copper is in an environment of four in-plane nitrogens with axial symmetry.

摘要

相似文献

1
An investigation of the role of the copper in galactose oxidase.
Biochemistry. 1975 Mar 25;14(6):1108-15. doi: 10.1021/bi00677a003.
2
[Fungal galactooxidase].[真菌半乳糖氧化酶]
Mikrobiol Zh (1978). 1979 Sep-Oct;41(5):560-9.
3
Circular dichroism spectra of the copper enzyme, galactose oxidase, in the presence of its substrates and products.在其底物和产物存在的情况下，铜酶半乳糖氧化酶的圆二色光谱。
Biochemistry. 1974 Mar 12;13(6):1247-51. doi: 10.1021/bi00703a030.
4
Kinetic and magnetic resonance studies of substrate binding to galactose oxidase copper(II).
J Inorg Biochem. 1981 Jun;14(3):223-35. doi: 10.1016/s0162-0134(00)80002-1.
5
Role of tryptophan in the spectral and catalytic properties of the copper enzyme, galactose oxidase.色氨酸在铜酶半乳糖氧化酶的光谱和催化特性中的作用。
Biochemistry. 1977 Apr 19;16(8):1597-601. doi: 10.1021/bi00627a011.
6
Histidine as an essential residue in the active site of the copper enzyme galactose oxidase.组氨酸作为铜酶半乳糖氧化酶活性位点中的必需残基。
Arch Biochem Biophys. 1977 Aug;182(2):712-22. doi: 10.1016/0003-9861(77)90553-7.
7
The involvement of superoxide and trivalent copper in the galactose oxidase reaction.超氧化物和三价铜在半乳糖氧化酶反应中的作用。
Adv Exp Med Biol. 1976;74:489-504. doi: 10.1007/978-1-4684-3270-1_42.
8
The valence of copper and the role of superoxide in the D-galactose oxidase catalyzed reaction.铜的化合价及超氧化物在D-半乳糖氧化酶催化反应中的作用。
Biochem Biophys Res Commun. 1973 Nov 16;55(2):333-40. doi: 10.1016/0006-291x(73)91092-9.
9
Fluorescence properties of the copper enzyme galactose oxidase and its tryptophan-modified derivatives.
Biochemistry. 1977 Apr 19;16(8):1602-6. doi: 10.1021/bi00627a012.
10
The reaction of nitric oxide with copper proteins and the photodissociation of copper-NO complexes.一氧化氮与铜蛋白的反应及铜-一氧化氮配合物的光解离
Biochim Biophys Acta. 1987 Nov 5;916(1):38-47. doi: 10.1016/0167-4838(87)90208-1.

引用本文的文献

1
Hematin and Cu control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion.血红素和铜控制过氧化氢自由基和超氧阴离子自由基的生成，以激活半乳糖氧化酶用于5-羟甲基糠醛转化。
iScience. 2023 Nov 17;26(12):108452. doi: 10.1016/j.isci.2023.108452. eCollection 2023 Dec 15.
2
Identification of Copper-Containing Oxidoreductases in the Secretomes of Three Species with a Focus on Copper Radical Oxidases for the Biocatalytic Production of Fatty Aldehydes.鉴定三种物种分泌组中的含铜氧化还原酶，重点是铜自由基氧化酶，用于生物催化生产脂肪醛。
Appl Environ Microbiol. 2021 Nov 24;87(24):e0152621. doi: 10.1128/AEM.01526-21. Epub 2021 Oct 6.
3
Formation of Monofluorinated Radical Cofactor in Galactose Oxidase through Copper-Mediated C-F Bond Scission.
通过铜介导的 C-F 键断裂在半乳糖氧化酶中形成单氟化自由基辅因子。
J Am Chem Soc. 2020 Nov 4;142(44):18753-18757. doi: 10.1021/jacs.0c08992. Epub 2020 Oct 22.
4
Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli.黑穗状镰刀菌半乳糖氧化酶在大肠杆菌中的表达、纯化及特性分析
Protein Expr Purif. 2015 Apr;108:73-79. doi: 10.1016/j.pep.2014.12.010. Epub 2014 Dec 24.
5
A dual staining method for identifying mucins of different gastric epithelial mucous cells.一种用于鉴定不同胃上皮黏液细胞黏蛋白的双重染色方法。
Histochem J. 1991 Jan;23(1):22-8. doi: 10.1007/BF01886504.