Chow M P, Sun K J, Yung C H, Hu H Y, Tzeng J L, Lee T D
Department of Medicine, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Acta Haematol. 1992;87(3):153-5. doi: 10.1159/000204744.
A male, full-term baby with thrombocytopenia was born by a G3P2A1 mother who was not associated with autoimmune disease. Platelet antibody screening was positive by using lymphocytotoxicity test, platelet suspension immunofluorescence test and solid-phase red cell adherence test. The identified HLA antibody was of A2 specificity. It was confirmed by testing the mother's and the baby's sera against the lymphocytes and platelets of 10 HLA-A2-positive donors. The possibility of platelet-specific antibody as the cause of neonatal alloimmune thrombocytopenia was ruled out by testing against platelets of 10 HLA-A2-negative donors and the known platelet-specific antigens utilizing immobilized, purified platelet glycoprotein as targets. The mother's serum reacted strongly with both the father's and the baby's platelets and lymphocytes. This neonatal thrombocytopenia was most likely due to the maternal HLA antibody, which was induced by her antecedent gestations.
一名足月男婴患有血小板减少症，其母亲为G3P2A1，无自身免疫性疾病。通过淋巴细胞毒性试验、血小板悬液免疫荧光试验和固相红细胞粘附试验，血小板抗体筛查呈阳性。鉴定出的HLA抗体具有A2特异性。通过检测母亲和婴儿的血清与10名HLA - A2阳性供者的淋巴细胞和血小板，得以证实。通过检测针对10名HLA - A2阴性供者的血小板以及利用固定化纯化血小板糖蛋白作为靶点的已知血小板特异性抗原，排除了血小板特异性抗体作为新生儿同种免疫性血小板减少症病因的可能性。母亲的血清与父亲和婴儿的血小板及淋巴细胞均发生强烈反应。这种新生儿血小板减少症很可能是由母亲先前妊娠诱导产生的母体HLA抗体所致。
