Riegler J, Ehlert O, Nann T
Freiburg Materials Research Center (FMF), Stefan-Meier-Strasse 21, 79104, Freiburg, Germany.
Anal Bioanal Chem. 2006 Feb;384(3):645-50. doi: 10.1007/s00216-005-0244-x. Epub 2006 Jan 19.
The present paper describes a new, facile and fast technique that can be used to incorporate CdSe/ZnS core/shell nanocrystals into polystyrene beads. The nanocrystals were immobilized within the polymer beads by demixing two nonmiscible solvents. The resulting beads were bright, homogeneously coded, and did not show any surface texture or photobleaching under application-relevant conditions. The beads were subsequently used on a model streptavidin-biotin binding system in order to demonstrate the applicability of the technique to enzyme-linked assays and multiplexing applications.
本文描述了一种新型、简便且快速的技术,该技术可用于将CdSe/ZnS核壳纳米晶体掺入聚苯乙烯珠中。通过使两种不混溶的溶剂分层,将纳米晶体固定在聚合物珠内。所得的珠子明亮、编码均匀,并且在与应用相关的条件下未显示出任何表面纹理或光漂白现象。随后,将这些珠子用于模型链霉亲和素-生物素结合系统,以证明该技术在酶联测定和多重应用中的适用性。