一种用于带有多色量子点的微珠的可行且定量的编码方法。
A feasible and quantitative encoding method for microbeads with multicolor quantum dots.
作者信息
Wang Hai-Qiao, Huang Zhen-Li, Liu Tian-Cai, Wang Jian-Hao, Cao Yuan-Cheng, Hua Xiao-Feng, Li Xiu-Qing, Zhao Yuan-Di
机构信息
Key Laboratory of Biomedical Photonics of Ministry of Education-Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan, HuBei, 430074 PR China.
出版信息
J Fluoresc. 2007 Mar;17(2):133-8. doi: 10.1007/s10895-007-0157-5. Epub 2007 Feb 8.
Multicolor encoded beads were achieved by incorporating two color core-shell quantum dots (QDs) (CdSe/ZnS) to commercial polystyrene (PS) beads. By controlling the concentration ratios of the two quantum dots (QDs) in doping solutions, a series of codes with different intensity ratios were obtained. Based on the multiple encoded carboxylic modified polystyrene beads, fluorescent dyes labeled antibodies were distinguished successfully on the beads' surface. It suggests that the encoded beads from this method have the practicability in biological applications and chemical analysis.
通过将两种颜色的核壳量子点（QDs）（CdSe/ZnS）掺入商业聚苯乙烯（PS）微珠中，制备了多色编码微珠。通过控制掺杂溶液中两种量子点（QDs）的浓度比，获得了一系列具有不同强度比的编码。基于多重编码的羧基修饰聚苯乙烯微珠，成功区分了微珠表面荧光染料标记的抗体。这表明该方法制备的编码微珠在生物应用和化学分析中具有实用性。
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