Lin H J, Han C Y, Nienhuis A W
Department of Pediatrics, Harbor-UCLA Medical Center, Torrance 90502.
Am J Hum Genet. 1992 Aug;51(2):363-70.
We performed a systematic functional analysis of the human gamma-globin promoter to identify its activator domains. We used a panel of truncation and scanning mutants as well as transfection in human K562 fetal erythroid cells. The various mutations produced relatively small changes in promoter function in both transient and stable transfection assays. The CACCC region and the region containing the binding sites for protein GATA-1 behaved as activator domains. We also obtained evidence for a minor activator site in the - 200 to - 190 region. The results are consistent with the interpretation that gamma-globin gene regulation may occur in part through multiple small effects of promoter elements.
我们对人γ-珠蛋白启动子进行了系统的功能分析，以确定其激活结构域。我们使用了一系列截短和扫描突变体，并在人K562胎儿红系细胞中进行转染。在瞬时和稳定转染实验中，各种突变在启动子功能上产生了相对较小的变化。CACCC区域和包含蛋白GATA-1结合位点的区域表现为激活结构域。我们还获得了在-200至-190区域存在一个次要激活位点的证据。这些结果与γ-珠蛋白基因调控可能部分通过启动子元件的多种小效应发生的解释一致。
