Lu Jian-Wei, Gao Chang-Ming, Wu Jian-Zhong, Cao Hai-Xia, Tajima Kazuo, Feng Ji-Feng
Department of Medical Oncology, Jiangsu Province Institute of Cancer Research, Nanjing, 210009, PR China.
Department of Epidemiology, Jiangsu Province Institute of Cancer Research, Nanjing, 210009, PR China.
J Hum Genet. 2006;51(3):155-160. doi: 10.1007/s10038-005-0339-4. Epub 2006 Jan 20.
To investigate the relationship between polymorphism in the 3'-untranslated region (3'-UTR) of the thymidylate synthase (TS) gene and sensitivity of gastric cancer to 5-fluorouracil (5-FU)-based chemotherapy, 106 cases of advanced gastric cancer were analyzed. All patients were treated with 5-FU-based chemotherapy; DNA from peripheral blood leukocytes was obtained before therapy. TS 3'-UTR genotypes were detected by PCR-RFLP. Polymorphism in the TS 3'-UTR can be classified into three groups according to the presence or absence of a 6 bp nucleotide fragment: the -6/-6 bp, -6/+6 bp and +6/+6 bp groups. The response rate of the -6/-6 bp and -6/+6 bp groups was found to be significantly higher than the +6/+6 bp group. These results show that the presence of the TS 3'-UTR 6 bp nucleotide fragment can be correlated with the sensitivity of gastric cancer to 5-FU-based chemotherapy, and that the TS 3'-UTR polymorphism profile can be used to guide the choice of 5-FU-based chemotherapy in advanced gastric cancer.
为了研究胸苷酸合成酶（TS）基因3'-非翻译区（3'-UTR）的多态性与胃癌对基于5-氟尿嘧啶（5-FU）化疗的敏感性之间的关系，分析了106例晚期胃癌患者。所有患者均接受基于5-FU的化疗；治疗前采集外周血白细胞的DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测TS 3'-UTR基因型。根据6 bp核苷酸片段的有无，TS 3'-UTR多态性可分为三组：-6/-6 bp、-6/+6 bp和+6/+6 bp组。发现-6/-6 bp和-6/+6 bp组的缓解率显著高于+6/+6 bp组。这些结果表明，TS 3'-UTR 6 bp核苷酸片段的存在可能与胃癌对基于5-FU化疗的敏感性相关，并且TS 3'-UTR多态性图谱可用于指导晚期胃癌基于5-FU化疗的选择。
