Lin Hai-Yan, Qian Dong, Zhang Xuan, Liu Guo-Yi, Wang Hong-Mei, Zhu Cheng
State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Sciences, 25 Bei Si Huan Xi Lu, Haidian District, Beijing 100080, PR China.
Life Sci. 2006 May 1;78(23):2669-75. doi: 10.1016/j.lfs.2005.10.012. Epub 2006 Jan 19.
Roles of transforming growth factor-beta (TGF-beta) receptor types I (TbetaRI) and II (TbetaRII) during the estrous cycle and implantation of rodents are currently unclear. In the present study, the spatial and temporal expressions of TbetaRI and TbetaRII in rat endometrium during the estrous cycle, pre-, and peri-implantation were examined using in situ hybridization and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). With in situ hybridization, TbetaRI and TbetaRII were expressed at weak levels in rat endometrium during the estrous cycle. During pre-implantation, both receptors were expressed in the luminal epithelium and glandular epithelium on Days 0.5 and 1.5 of pregnancy, but were down-regulated on Days 2.5 and 3.5. During peri-implantation, both TGF-beta receptors were localized in the luminal epithelium and subepithelial stroma to facilitate attachment reaction and trophoblast invasion. They were highly expressed on Day 4.5, whereas were down-regulated on Days 5.5 and 6.5. Semi-quantitative RT-PCR analysis confirmed the data obtained by in situ hybridization. These results suggest that during pre-implantation, both TGF-beta receptors are functional in the proliferation of endometrial epithelial cells. During peri-implantation, both TGF-beta receptors play important roles during the onset of the uterine receptivity and the attachment reaction. TGF-beta signaling is down-regulated when trophoblast invasion begins.
转化生长因子-β（TGF-β）Ⅰ型受体（TβRI）和Ⅱ型受体（TβRII）在啮齿动物发情周期和着床过程中的作用目前尚不清楚。在本研究中，我们使用原位杂交和半定量逆转录-聚合酶链反应（RT-PCR）检测了发情周期、着床前和着床周围时期大鼠子宫内膜中TβRI和TβRII的时空表达。原位杂交结果显示，发情周期期间大鼠子宫内膜中TβRI和TβRII表达较弱。在着床前，妊娠第0.5天和1.5天，两种受体均在腔上皮和腺上皮中表达，但在第2.5天和3.5天表达下调。在着床周围时期，两种TGF-β受体均定位于腔上皮和上皮下基质，以促进黏附反应和滋养层侵入。它们在第4.5天高度表达，而在第5.5天和6.5天表达下调。半定量RT-PCR分析证实了原位杂交获得的数据。这些结果表明，在着床前，两种TGF-β受体在子宫内膜上皮细胞增殖中发挥作用。在着床周围时期，两种TGF-β受体在子宫接受性开始和黏附反应中起重要作用。当滋养层侵入开始时，TGF-β信号通路下调。
