Choi Kyoung-Soo, Song Liguo, Park Young-Mee, Marshall James, Lund Anders L, Shion Henry, Park Eun-Mi, Chae Ho-Zoon, Park Jong Hoon
Department of Cellular Stress Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Prep Biochem Biotechnol. 2006;36(1):3-17. doi: 10.1080/10826060500388447.
We compared the 2DE coupled to MALDI-TOF-MS and ESI-MS/MS analysis (2DE-MS) and the on-line 2D nanoLC, followed by nanoESI-MS/MS analysis (2DLC-MS), for the separation and identification of proteins in high abundance protein-depleted human plasma. Identification of proteins in the plasma by the two methods demonstrated that the majority of the identified protein set was unique to each method. Therefore, if a comprehensive coverage of the proteome identification is desired, it is ideal to apply both methods. The 2DE-MS method is amenable to protein spot-based quantitation, whereas the 2DLC-MS method may provide an advantage of the high throughput application.
我们比较了二维凝胶电泳（2DE）结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和电喷雾串联质谱（ESI-MS/MS）分析（2DE-MS）以及在线二维纳升级液相色谱（2D nanoLC），随后进行纳升电喷雾串联质谱（nanoESI-MS/MS）分析（2DLC-MS），用于分离和鉴定去除高丰度蛋白的人血浆中的蛋白质。通过这两种方法对血浆中的蛋白质进行鉴定表明，大多数鉴定出的蛋白质集对于每种方法来说都是独特的。因此，如果希望全面覆盖蛋白质组鉴定，同时应用这两种方法是理想的选择。2DE-MS方法适用于基于蛋白质斑点的定量分析，而2DLC-MS方法可能具有高通量应用的优势。
