Sagawa Norifumi, Takino Takashi, Kurogochi Shin
Yuki Research Center, Bayer CropScience Co., Ltd, Yuki, Ibaraki, Japan.
Biosci Biotechnol Biochem. 2006 Jan;70(1):230-6. doi: 10.1271/bbb.70.230.
A selective and speedy LC-MS/MS method was developed to determine six trichothecene mycotoxins (nivalenol, deoxynivalenol, fusarenon X, 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, and T-2 toxin) in rice medium where Fusarium graminearum were cultivated for in vitro tests. The analytes were extracted from the rice medium with acetonitrile/water (85/15, v/v), and diluted with acetonitrile/water (5/95, v/v) in order to minimize the effects of matrices. Diluted solutions were analyzed by LC-MS/MS with electrospray ionization (ESI) interface in negative or positive ion mode and the multiple reaction monitoring mode. Recovery rates were 76-106% with a spiked level at 1-6 microg/kg of mycotoxins that corresponded to the limit of quantitation. The method was applied to study the time courses of trichothecene production and the biomass of fungi by three Fusarium graminearum strains. Three strains have different mycotoxin biosynthesis pathways, wFg14 and 03E-1 were DON producer, and 03N-1 was NIV producer.
建立了一种选择性快速的液相色谱-串联质谱（LC-MS/MS）方法，用于测定在用于体外试验的水稻培养基中培养禾谷镰刀菌时的六种单端孢霉烯族霉菌毒素（雪腐镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、镰刀菌烯酮X、15-乙酰脱氧雪腐镰刀菌烯醇、3-乙酰脱氧雪腐镰刀菌烯醇和T-2毒素）。用乙腈/水（85/15，v/v）从水稻培养基中提取分析物，并用乙腈/水（5/95，v/v）稀释，以尽量减少基质的影响。稀释后的溶液通过带有电喷雾电离（ESI）接口的LC-MS/MS在负离子或正离子模式以及多反应监测模式下进行分析。在1-6μg/kg霉菌毒素的加标水平下（对应定量限），回收率为76-106%。该方法用于研究三种禾谷镰刀菌菌株产生单端孢霉烯族毒素的时间进程和真菌生物量。三种菌株具有不同的霉菌毒素生物合成途径，wFg14和03E-1是脱氧雪腐镰刀菌烯醇产生菌，03N-1是雪腐镰刀菌烯醇产生菌。
