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Purification of BrdUrd-substituted DNA by immunoaffinity chromatography with anti-BrdUrd antibodies.

作者信息

Contreas G, Giacca M, Falaschi A

机构信息

International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.

出版信息

Biotechniques. 1992 Jun;12(6):824-6.

PMID:1642886
Abstract
摘要

相似文献

1
Purification of BrdUrd-substituted DNA by immunoaffinity chromatography with anti-BrdUrd antibodies.使用抗溴脱氧尿苷抗体通过免疫亲和色谱法纯化溴脱氧尿苷取代的DNA。
Biotechniques. 1992 Jun;12(6):824-6.
2
Application of an ELISA procedure for the quantitation of bromodeoxyuridine incorporated into cellular DNA.
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Interstrand cross-link induction by UV radiation in bromodeoxyuridine-substituted DNA: dependence on DNA conformation.紫外线辐射在溴脱氧尿苷取代的DNA中诱导链间交联：对DNA构象的依赖性。
Biochemistry. 2005 Dec 27;44(51):16957-66. doi: 10.1021/bi050799x.
4
[Flow cytometric dual-parameter analysis of human leukemic cells using monoclonal anti-BrdUrd antibody].
Gan No Rinsho. 1985 May;31(5):549-51.
5
Flow cytometric estimation of cell cycle parameters using a monoclonal antibody to bromodeoxyuridine.使用抗溴脱氧尿苷单克隆抗体通过流式细胞术评估细胞周期参数。
Cytometry. 1986 Jul;7(4):391-5. doi: 10.1002/cyto.990070415.
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Flow cytometric measurement of RNA synthesis using bromouridine labelling and bromodeoxyuridine antibodies.使用溴尿苷标记和溴脱氧尿苷抗体通过流式细胞术测量RNA合成。
Cytometry. 1993;14(4):455-8. doi: 10.1002/cyto.990140416.
7
Effect of 5-fluoro-2'-deoxyuridine (FdUrd) on 5-bromo-2'-deoxyuridine (BrdUrd) incorporation into DNA measured with a monoclonal BrdUrd antibody and by the BrdUrd/Hoechst quenching effect.5-氟-2'-脱氧尿苷（FdUrd）对5-溴-2'-脱氧尿苷（BrdUrd）掺入DNA的影响，通过单克隆BrdUrd抗体及BrdUrd/ Hoechst淬灭效应进行测定。
Cytometry. 1985 Nov;6(6):513-20. doi: 10.1002/cyto.990060605.
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Detection of 5-bromo-2-deoxyuridine (BrdUrd) incorporation with monoclonal anti-BrdUrd antibody after deoxyribonuclease treatment.
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Synergistic cytotoxicity and DNA strand break formation by bromodeoxyuridine and bleomycin in human tumor cells.
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Automated two-column purification of iminobiotin and BrdU-labeled PCR products for rapid cloning: application to genes synthesized by polymerase chain assembly.用于快速克隆的亚氨基生物素和BrdU标记的PCR产物的自动双柱纯化：应用于通过聚合酶链组装合成的基因
J Chromatogr Sci. 2010 Feb;48(2):120-4. doi: 10.1093/chromsci/48.2.120.

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Replication of the chicken beta-globin locus: early-firing origins at the 5' HS4 insulator and the rho- and betaA-globin genes show opposite epigenetic modifications.鸡β-珠蛋白基因座的复制：5' HS4绝缘子以及rho-和βA-珠蛋白基因处的早期激活起始位点呈现相反的表观遗传修饰。
Mol Cell Biol. 2003 May;23(10):3536-49. doi: 10.1128/MCB.23.10.3536-3549.2003.
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Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone.仓鼠二氢叶酸还原酶基因起始区域中DNA复制主要起始位点的鉴定
Mol Cell Biol. 1998 Jun;18(6):3266-77. doi: 10.1128/MCB.18.6.3266.
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oriGNAI3: a narrow zone of preferential replication initiation in mammalian cells identified by 2D gel and competitive PCR replicon mapping techniques.
oriGNAI3：通过二维凝胶和竞争性PCR复制子定位技术鉴定出的哺乳动物细胞中优先复制起始的狭窄区域。
Nucleic Acids Res. 1998 May 15;26(10):2313-21. doi: 10.1093/nar/26.10.2313.
4
Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex.人类主要组织相容性复合体中GC含量过渡区域DNA复制时间的精确切换。
Mol Cell Biol. 1997 Jul;17(7):4043-50. doi: 10.1128/MCB.17.7.4043.
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High-resolution mapping of the origin of DNA replication in the hamster dihydrofolate reductase gene domain by competitive PCR.通过竞争性PCR对仓鼠二氢叶酸还原酶基因区域中DNA复制起点进行高分辨率定位。
Mol Cell Biol. 1996 Oct;16(10):5358-64. doi: 10.1128/MCB.16.10.5358.
6
Utilization of the same DNA replication origin by human cells of different derivation.不同来源的人类细胞对相同DNA复制起点的利用。
Nucleic Acids Res. 1996 Sep 1;24(17):3289-94. doi: 10.1093/nar/24.17.3289.
7
Fine mapping of a replication origin of human DNA.人类DNA复制起点的精细定位
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7119-23. doi: 10.1073/pnas.91.15.7119.
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The quest for a human ori.对人类复制起点的探索。
Genetica. 1994;94(2-3):255-66. doi: 10.1007/BF01443439.
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The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.一种新型人类核纤层蛋白的基因定位于19号染色体的一个高转录位点，该位点在S期开始时进行复制。
Mol Cell Biol. 1992 Aug;12(8):3499-506. doi: 10.1128/mcb.12.8.3499-3506.1992.
10
A human DNA replication origin: localization and transcriptional characterization.一个人类DNA复制起点：定位与转录特征分析
Chromosoma. 1992;102(1 Suppl):S24-31. doi: 10.1007/BF02451782.